成人欧美一区二区三区,天天干天天操天天爱,免费特黄一级欧美大片在线看 ,亚洲中文字幕无码一区日日添

    <span id="alen4"></span>

    <li id="alen4"></li>

      首頁/ 產品解讀 / 新聞詳情

      CCK-8,科研實驗中最常見的細胞增殖檢測方法

      CCK-8法目前是生命科學研究中檢測細胞增殖和毒性應用最廣泛的方法之一,截至2022年,在Pubmed數據庫中利用CCK-8試劑發(fā)表的文獻數逐年遞增。
      圖片
       
       

      實驗原理

       圖片

      細胞增殖的檢測方法眾多,CCK-8是細胞增殖檢測中的代謝活性檢測,屬于MTT的升級產品,操作較MTT更便捷(不需要DMSO溶解)。

       

      工作原理:CCK-8可以被細胞線粒體內的脫氫酶還原生成高度水溶性的橙黃色的甲臜產物。顏色的深淺與細胞的增殖成正比,與細胞毒性成反比。使用酶標儀在450nm波長處測定OD值,間接反映活細胞數量。

       

       

       

      實驗流程和操作步驟

      圖片
       
       

      01

      實驗分組(每組建議3-6個復孔)

      實驗組:藥物或者基因過表達等處理細胞+CCK-8試劑(見下表黃色列)
      對照組:不加藥物等的對照細胞+CCK-8試劑(見下表綠色列)
      空白組:不加細胞的空白培養(yǎng)基+CCK-8試劑(見下表藍色列)
      PBS孔:灰色,防止液體過度蒸發(fā)

       

      排版參考:
      圖片

       

      02

      實驗方案

      1.種板細胞數:
       
      細胞增殖實驗:每孔加入約1000-2000個細胞,100μl培養(yǎng)基,每0,24,48,72h檢測細胞的OD值。

       

      細胞毒性實驗:每孔加入約5000~10000個細胞,100μl培養(yǎng)基(具體每孔所用的細胞的數目,需根據細胞的大小,細胞增殖速度的快慢等決定)??装逯車?00μL PBS防止過度蒸發(fā)。

       

      2.檢測方法
      在對應的檢測時間點,從培養(yǎng)箱取出細胞,每孔加入10μL的CCK-8試劑,避光反映1-4h(具體孵育時間根據細胞數決定,一般OD值在0.8-1.5時線性最佳),在酶標儀上450nm下檢測OD值,檢測前需震蕩混勻。

       

      注:換液和不換液均可。若檢測完以后第二天還需要再一次檢測,則全部吸出黃色反應液,再加入新鮮培養(yǎng)基培養(yǎng)細胞。

       

       

      結果分析

      我們得到的值是酶標儀讀出的OD值,一般結果可用OD值呈現(xiàn),也可采用處理后的細胞存活率呈現(xiàn)。細胞存活率% =(加藥細胞OD-空白OD) /(對照細胞OD-空白OD)×100%,可用GraphPad軟件作出相應圖片。

       

      文獻示例一——計算細胞存活率

      橫坐標:細胞組別;縱坐標,細胞存活率(YEASEN貨號:40203)
      圖片
       
      圖片參考文獻:
      Wei S, Zhao Q, Zheng K, et al. GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell Discov. 2022;8(1):77. Published 2022 Aug 9. doi:10.1038/s41421-022-00423-0(IF:38.079)

       

      文獻示例二——直接展示細胞OD值

      橫坐標:細胞生長時間;縱坐標:OD值(YEASEN貨號:40203)
      圖片

       

      圖片參考文獻:
      Han BY, Liu Z, Hu X, Ling H. HNRNPU promotes the progression of triple-negative breast cancer via RNA transcription and alternative splicing mechanisms. Cell Death Dis. 2022 Nov 8;13(11):940. doi: 10.1038/s41419-022-05376-6. (IF:5.9590)

       

      產品推薦

       
      產品名稱 產品編號 規(guī)格
      Cell Counting Kit (CCK-8) CCK-8試劑盒 40203ES60 100T
      40203ES76 500T
      40203ES80 1000T
      40203ES88 3×1000T
      40203ES92 10×1000T

       

      引用YEASEN產品部分發(fā)表文獻參考

      滑動查看

      [1] Sun L, Li P, Ju X, et al. In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Cell. 2021;184(7):1865-1883.e20. doi:10.1016/j.cell.2021.02.008(IF:41.584)

      [2] Wei S, Zhao Q, Zheng K, et al. GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell Discov. 2022;8(1):77. Published 2022 Aug 9. doi:10.1038/s41421-022-00423-0(IF:38.079)

      [3] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293. doi:10.1038/s41587-019-0249-1(IF:31.864)

      [4] Yang F, Xiao Y, Ding JH, et al. Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy [published online ahead of print, 2022 Oct 11]. Cell Metab. 2022;S1550-4131(22)00411-9. doi:10.1016/j.cmet.2022.09.021(IF:31.373)

      [5] Rong QX, Wang F, Guo ZX, et al. GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Mol Cancer. 2021;20(1):80. Published 2021 May 29. doi:10.1186/s12943-021-01374-y(IF:27.401)

      [6] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)

      [7] Yang X, Zhao X, Zhu Y, et al. FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation. Emerg Microbes Infect. 2022;11(1):2785-2799. doi:10.1080/22221751.2022.2140605(IF:19.568)

      [8] Zhou Z, Zhang X, Lei X, et al. Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Signal Transduct Target Ther. 2021;6(1):382. Published 2021 Nov 3. doi:10.1038/s41392-021-00800-3(IF:18.187)

      [9] Li M, Hao B, Zhang M, et al. Melatonin enhances radiofrequency-induced NK antitumor immunity, causing cancer metabolism reprogramming and inhibition of multiple pulmonary tumor development. Signal Transduct Target Ther. 2021;6(1):330. Published 2021 Sep 1. doi:10.1038/s41392-021-00745-7(IF:18.187)

      [10] Qi S, Zhu Y, Liu X, et al. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy. Mol Cell. 2022;82(10):1850-1864.e7. doi:10.1016/j.molcel.2022.03.027(IF:17.970)

      [11] Zhu J, Li X, Cai X, et al. Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity. Mol Cell. 2021;81(15):3171-3186.e8. doi:10.1016/j.molcel.2021.06.004(IF:17.970)

      [12] Teng KX, Niu LY, Xie N, Yang QZ. Supramolecular photodynamic agents for simultaneous oxidation of NADH and generation of superoxide radical. Nat Commun. 2022;13(1):6179. Published 2022 Oct 19. doi:10.1038/s41467-022-33924-3(IF:17.694)

      [13] Zhong J, Guo Y, Lu S, et al. Rational design of a sensitivity-enhanced tracer for discovering efficient APC-Asef inhibitors. Nat Commun. 2022;13(1):4961. Published 2022 Aug 24. doi:10.1038/s41467-022-32612-6(IF:17.694)

      [14] Liu F, Wang X, Duan J, et al. A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells. Adv Sci (Weinh). 2022;9(22):e2104823. doi:10.1002/advs.202104823(IF:16.806)

      [15] Ji C, Qiu M, Ruan H, et al. Transcriptome Analysis Revealed the Symbiosis Niche of 3D Scaffolds to Accelerate Bone Defect Healing. Adv Sci (Weinh). 2022;9(8):e2105194. doi:10.1002/advs.202105194(IF:16.806)

      [16] Feng L, Dou C, Xia Y, et al. Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery. ACS Nano. 2021;15(2):2263-2280. doi:10.1021/acsnano.0c07973(IF:15.881)

      [17] Wang Z, Gong X, Li J, et al. Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)

      [18] Jiang Z, He L, Yu X, et al. Antiangiogenesis Combined with Inhibition of the Hypoxia Pathway Facilitates Low-Dose, X-ray-Induced Photodynamic Therapy [published online ahead of print, 2021 Jun 25]. ACS Nano. 2021;10.1021/acsnano.1c01063. doi:10.1021/acsnano.1c01063(IF:15.881)

      [19] Gong X, Li J, Xu X, et al. Microvesicle-inspired oxygen-delivering nanosystem potentiates radiotherapy-mediated modulation of tumor stroma and antitumor immunity. Biomaterials. 2022;290:121855. doi:10.1016/j.biomaterials.2022.121855(IF:15.304)

      [20] Deng J, Xu W, Lei S, et al. Activated Natural Killer Cells-Dependent Dendritic Cells Recruitment and Maturation by Responsive Nanogels for Targeting Pancreatic Cancer Immunotherapy. Small. 2022;18(44):e2203114. doi:10.1002/smll.202203114(IF:15.153)

       

       

      400-6111-883