Recombinant Human HLA-A*11:01&B2M&KRAS G12S (VVVGASGVGK) Tetramer Protein,His-Avi Tag
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性能參數(shù)
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分子別名（Synonyms） |
K-Ras 2;C-K-RAS;KRAS;MHC |
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Human HLA-A*11:01&B2M&KRAS G12S (VVVGASGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGASGVGK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGASGVGK] |
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分子量大?。∕olecular Weight） |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on SDS-PAGE result. |
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內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC. |
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制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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重構(gòu)方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲(chǔ)存條件
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項(xiàng)
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
