蛋白別名（Synonyms）

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-汕; PTGF-汕; PTGFBPTGF-汕

種屬（Species)

Human

表達(dá)系統(tǒng)（Source）

E.coli

表達(dá)序列（Sequence）

Ala197-Ile308

Accession

Q99988-1

標(biāo)簽（Tag)

N-His

分子量（Molecular Weight）

The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

內(nèi)毒素（Endotoxin）

< 1 EU/μg by the LAL method.

純度（Purity）

> 95% as determined by Bis-Tris PAGE

活性（Activity）

Blocking Data: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
ELISA Data: Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA .
SPR Data: Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.

外觀（Appearance）

凍干粉