產(chǎn)品描述
R-Spondin-1(Rspo-1)屬于Wnt調(diào)節(jié)劑的(Rspo)家族。目前,該家族由四個結(jié)構(gòu)相關(guān)的分泌配體(Rspo 1-4)組成,所有配體都含有弗林樣結(jié)構(gòu)域和血小板反應(yīng)蛋白結(jié)構(gòu)域。Rspo-1在發(fā)育中的中樞神經(jīng)系統(tǒng)的某些區(qū)域以及腎上腺、卵巢、睪丸、甲狀腺和氣管中表達(dá)。Rspo可以以刺激Wnt/β-catenin信號通路的方式與Frizzled/LRP6受體復(fù)合物相互作用。隨著類器官領(lǐng)域的擴大,R-Spondin 1被廣泛用于類器官細(xì)胞培養(yǎng)中,作為促進(jìn)3D類器官生長和存活的重要成分。
R-性能參數(shù)
蛋白別名(Synonyms) |
R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 |
種屬(Species) |
Mouse |
表達(dá)系統(tǒng)(Source) |
HEK293 |
表達(dá)序列(Sequence) |
Ser21-Gln265 |
Accession |
Q9Z132 |
標(biāo)簽(Tag) |
C-His |
分子量(Molecular Weight) |
The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
內(nèi)毒素(Endotoxin) |
< 1 EU/μg by the LAL method. |
純度(Purity) |
> 95% as determined by Bis-Tris PAGE |
活性(Activity) |
ELISA Data: Immobilized Mouse R spondin 1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
the presence of 5 ng/mL recombinant Human Wnt3a . |
制劑(Formulation) |
Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
外觀(Appearance) |
凍干粉 |
儲存條件
-25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, 2 ~8 °C保存,7天有效期。復(fù)溶后, -85~-65℃保存,3個月有效期。
復(fù)溶方法
開蓋之前,先離心。建議復(fù)溶至濃度超過100μg/ml。將凍干的蛋白質(zhì)溶解在 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5)。
注意事項
1.為了您的安全和健康,請穿實驗服并戴一次性手套操作。
2.本產(chǎn)品僅作科研用途。
Ver. CN20240919