蛋白別名（Synonyms）

FL; Flt3 ligand; Flt3L; FLT3LG; SL cytokine

種屬（Species)

Mouse

表達(dá)系統(tǒng)（Source）

HEK293

表達(dá)序列（Sequence）

Gly27-Gln189

Accession

P49772-1

標(biāo)簽（Tag)

C-His

分子量（Molecular Weight）

The protein has a predicted MW of 19.61 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.

內(nèi)毒素（Endotoxin）

< 1 EU/μg by the LAL method.

純度（Purity）

> 95% as determined by Bis-Tris PAGE;  > 95% as determined by HPLC

活性（Activity）

ELISA Data: Immobilized Mouse FLT3 Ligand, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 39.3ng/ml determined by ELISA.

SPR Data: Mouse FLT3 Ligand, His Tag captured on CM5 Chip via anti-his antibody can bind Human FLT3, hFc Tag with an affinity constant of 0.18 nM as determined in SPR assay.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

外觀（Appearance）

凍干粉