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產(chǎn)品描述
載脂蛋白E(apoE)是外周和中樞神經(jīng)系統(tǒng)中的脂質(zhì)載體。載脂的apoE脂蛋白顆粒與多種細(xì)胞表面受體結(jié)合,以支持腦膜穩(wěn)態(tài)和損傷修復(fù)。考慮到患病率和相對(duì)風(fēng)險(xiǎn)大小,APOE基因的α4等位基因是晚發(fā)型阿爾茨海默?。ˋD)最強(qiáng)的遺傳風(fēng)險(xiǎn)因素。
性能參數(shù)
蛋白別名(Synonyms) |
APOE2; APOE; Apo-E |
種屬(Species) |
Human |
表達(dá)系統(tǒng)(Source) |
HEK293 |
表達(dá)序列(Sequence) |
Lys19-His317(R176C) |
Accession |
P02649-1 |
標(biāo)簽(Tag) |
C-His |
分子量(Molecular Weight) |
The protein has a predicted MW of 35.28 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Bis-Tris PAGE result. |
內(nèi)毒素(Endotoxin) |
< 1 EU/μg by the LAL method. |
純度(Purity) |
> 95% as determined by Bis-Tris PAGE; |
活性(Activity) |
SPR Data: Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE2, His Tag with an affinity constant of 27.11 nM as determined in SPR assay. |
制劑(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外觀(Appearance) |
凍干粉 |
儲(chǔ)存條件
-25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, 2 ~8 °C保存,7天有效期。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。
復(fù)溶方法
開蓋之前,先離心。使用蒸餾水復(fù)溶凍干的蛋白質(zhì),復(fù)溶濃度超過100 μg/mL。建議首次使用時(shí)將蛋白分裝為每次實(shí)驗(yàn)用量,避免反復(fù)凍融。
注意事項(xiàng)
1.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
2.本產(chǎn)品僅作科研用途。
Ver. CN20240919