產(chǎn)品簡介
白細(xì)胞介素-3(Interleukin-3,IL-3)是一種造血生長因子,可促進(jìn)巨核細(xì)胞、粒細(xì)胞-巨噬細(xì)胞、紅系、嗜酸性粒細(xì)胞、嗜堿性粒細(xì)胞和肥大細(xì)胞譜系的定向祖細(xì)胞的存活、分化和增殖。IL-3由T細(xì)胞、肥大細(xì)胞和嗜酸性粒細(xì)胞產(chǎn)生,可增強(qiáng)血小板生成素、吞噬作用和抗體介導(dǎo)的細(xì)胞毒性。其激活單核細(xì)胞的能力表明IL-3可能具有額外的免疫調(diào)節(jié)作用。許多IL-3活性依賴于與其他細(xì)胞因子的共同刺激。
重組人IL-3是一種15.0kDa的重組蛋白,含有133個(gè)氨基酸殘基。本品由大腸桿菌表達(dá),以凍干粉形式提供,高活性、高純度、低內(nèi)毒素、無標(biāo)簽。
性能參數(shù)
別名(Synonyms) |
Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
表達(dá)系統(tǒng)(Source) |
E.coli. |
表達(dá)序列(Sequence) |
Ala20-Phe152. |
分子量(Molecular Weight) |
Approximately 15.08 kDa. |
Accession |
P08700 |
標(biāo)簽(Tag) |
No Tag |
生物活性(Biological Activity) |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg. |
純度(Purity) |
> 95% as determined by SDS-PAGE. |
內(nèi)毒素(Endotoxin) |
< 0.01EU/μg protein as determined by LAL method. |
無菌(Sterility) |
Negative. |
支原體檢測(cè)(Mycoplasma) |
Negative. |
外源病毒(Vitro Virus) |
Negative. |
宿主殘留蛋白(Host Cell Protein) |
Less than 0.5 ng/ug when tested by ELISA. |
宿主殘留DNA(Host Cell DNA) |
Less than 20 ng/mg when tested by qPCR. |
制劑(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
外觀(Physical Appearance) |
Sterile filtered white lyophilized (freeze-dried) powder. |
儲(chǔ)存條件
-25~-15℃保存,有效期3年。
復(fù)溶后, 2 ~8 ℃保存,30天有效期。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。
復(fù)溶方法
建議將Human IL-3在無菌PBS中復(fù)溶至0.1-1.0 mg/ml的濃度。盡可能避免反復(fù)凍融。
注意事項(xiàng)
1.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
2.本產(chǎn)品僅作科研用途。
產(chǎn)品數(shù)據(jù)
SDS- PAGE
Human IL-3 on SDS-PAGE under reduced condition.
The purity is greater than 95%.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using human TF-1 cells
is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.
Ver. CN20241022