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      Recombinant Human IL-3 GMP Protein 重組人白介素3

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

      產(chǎn)品簡介

       

      白細(xì)胞介素-3Interleukin-3,IL-3是一種造血生長因子,可促進(jìn)巨核細(xì)胞、粒細(xì)胞-巨噬細(xì)胞、紅系、嗜酸性粒細(xì)胞、嗜堿性粒細(xì)胞和肥大細(xì)胞譜系的定向祖細(xì)胞的存活、分化和增殖。IL-3由T細(xì)胞、肥大細(xì)胞和嗜酸性粒細(xì)胞產(chǎn)生,可增強(qiáng)血小板生成素、吞噬作用和抗體介導(dǎo)的細(xì)胞毒性。其激活單核細(xì)胞的能力表明IL-3可能具有額外的免疫調(diào)節(jié)作用。許多IL-3活性依賴于與其他細(xì)胞因子的共同刺激。

      重組人IL-3是一種15.0kDa的重組蛋白,含有133個(gè)氨基酸殘基。本品由大腸桿菌表達(dá),以凍干粉形式提供,高活性、高純度、低內(nèi)毒素、無標(biāo)簽。

       

      性能參數(shù)

       

      別名(Synonyms

      Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor

      表達(dá)系統(tǒng)(Source

      E.coli.

      表達(dá)序列(Sequence

      Ala20-Phe152.

      分子量(Molecular Weight

      Approximately 15.08 kDa.

      Accession

      P08700

      標(biāo)簽(Tag)

      No Tag

      生物活性(Biological Activity

      The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.

      純度(Purity

      > 95% as determined by SDS-PAGE.

      內(nèi)毒素(Endotoxin

       0.01EU/μg protein as determined by LAL method.

      無菌(Sterility

      Negative.

      支原體檢測(cè)(Mycoplasma

      Negative.

      外源病毒(Vitro Virus

      Negative.

      宿主殘留蛋白(Host Cell Protein

      Less than 0.5 ng/ug when tested by ELISA.

      宿主殘留DNA(Host Cell DNA

      Less than 20 ng/mg when tested by qPCR.

      制劑(Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

      外觀(Physical Appearance

      Sterile filtered white lyophilized (freeze-dried) powder.

       

      儲(chǔ)存條件

       

      -25~-15℃保存,有效期3年。

      復(fù)溶后, 2 ~8 保存,30天有效期。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。

       

      復(fù)溶方法

       

      建議將Human IL-3在無菌PBS中復(fù)溶至0.1-1.0 mg/ml的濃度。盡可能避免反復(fù)凍融。

       

      注意事項(xiàng)

       

      1.為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

      2.本產(chǎn)品僅作科研用途。

       

      產(chǎn)品數(shù)據(jù)

       

      SDS- PAGE

       

      Human IL-3 on SDS-PAGE under reduced condition.

      The purity is greater than 95%.

       

      Cell Based Assay

      The ED50 as determined by a cell proliferation assay using human TF-1 cells

       is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.

       

      Ver. CN20241022

      400-6111-883