產(chǎn)品簡介
堿性成纖維細胞生長因子(bFGF)是成纖維細胞生長因子(FGF)家族的成員,是由FGF2基因編碼的生長因子和信號蛋白。FGF家族成員具有廣泛的促有絲分裂和細胞存活活性,并參與多種生物學過程,包括胚胎發(fā)育、細胞生長、形態(tài)發(fā)生、組織修復、腫瘤生長和侵襲。在正常組織中,bFGF存在于基底膜和血管內(nèi)皮下細胞外基質(zhì)中。bFGF能刺激和調(diào)節(jié)血管內(nèi)皮細胞、上皮細胞、成肌細胞、成骨細胞和神經(jīng)膠質(zhì)細胞等多種起源于中胚層、神經(jīng)外胚層的細胞分化增殖,在胚胎發(fā)育、組織愈合中起重要作用。bFGF/FGF-2,是所有胚胎和誘導多能干細胞培養(yǎng)基中維持多能性的基本成分。
本品由大腸桿菌表達,以液體形式提供,高活性、高純度、低內(nèi)毒素、無標簽。
性能參數(shù)
別名(Synonyms) |
bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin. |
表達系統(tǒng)(Source) |
Human bFGF Protein is expressed from E.coli without tag. |
表達序列(Sequence) |
Ala 144 - Ser 288. |
分子量(Molecular Weight) |
Approximately 16.29 kDa. |
Accession |
P09038. |
生物活性(Biological Activity) |
The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
純度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
內(nèi)毒素(Endotoxin) |
<0.01EU/μg protein as determined by LAL method. |
無菌(Sterility) |
Negative. |
支原體檢測(Mycoplasma) |
Negative. |
外源病毒(Vitro Virus) |
Negative. |
宿主殘留蛋白(Host Cell Protein) |
Less than 0.5 ng/ug when tested by ELISA. |
宿主殘留DNA(Host Cell DNA) |
Less than 20 ng/mg when tested by qPCR. |
制劑(Formulation) |
Liquuid from 0.22 μm filtered solution in PBS. |
外觀(Physical Appearance) |
Liquid. |
儲存條件
-85~-65℃保存,有效期2年。
注意事項
1.為了您的安全和健康,請穿實驗服并戴一次性手套操作。
2.本產(chǎn)品僅作科研用途。
產(chǎn)品數(shù)據(jù)
SDS-PAGE
Human FGF basic/FGF2/bFGF on SDS-PAGE under reduced condition.
The purity is greater than 95%.
SEC-HPLC
The purity of Human bFGF is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is
less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Ver. CN20241030