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      Recombinant Human FGF basic/FGF2/bFGF GMP Protein 重組人堿性成纖維細胞生長因子(GMP級)

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      產(chǎn)品簡介

       

      堿性成纖維細胞生長因子(bFGF是成纖維細胞生長因子(FGF)家族的成員,是由FGF2基因編碼的生長因子和信號蛋白。FGF家族成員具有廣泛的促有絲分裂和細胞存活活性,并參與多種生物學過程,包括胚胎發(fā)育、細胞生長、形態(tài)發(fā)生、組織修復、腫瘤生長和侵襲。在正常組織中,bFGF存在于基底膜和血管內(nèi)皮下細胞外基質(zhì)中。bFGF能刺激和調(diào)節(jié)血管內(nèi)皮細胞、上皮細胞、成肌細胞、成骨細胞和神經(jīng)膠質(zhì)細胞等多種起源于中胚層、神經(jīng)外胚層的細胞分化增殖,在胚胎發(fā)育、組織愈合中起重要作用。bFGF/FGF-2,是所有胚胎和誘導多能干細胞培養(yǎng)基中維持多能性的基本成分。

      本品由大腸桿菌表達,液體形式提供,高活性、高純度、低內(nèi)毒素、無標簽。

       

      性能參數(shù)

       

      別名(Synonyms

      bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin.

      表達系統(tǒng)(Source

      Human bFGF Protein is expressed from E.coli without tag.

      表達序列(Sequence

      Ala 144 - Ser 288.

      分子量(Molecular Weight

      Approximately 16.29 kDa.

      Accession

      P09038.

      生物活性(Biological Activity

      The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.

      純度(Purity

      > 95% as determined by SDS-PAGE and HPLC.

      內(nèi)毒素(Endotoxin

      0.01EU/μg protein as determined by LAL method.

      無菌(Sterility

      Negative.

      支原體檢測(Mycoplasma

      Negative.

      外源病毒(Vitro Virus

      Negative.

      宿主殘留蛋白(Host Cell Protein

      Less than 0.5 ng/ug when tested by ELISA.

      宿主殘留DNA(Host Cell DNA

      Less than 20 ng/mg when tested by qPCR.

      制劑(Formulation

      Liquuid from 0.22 μm filtered solution in PBS.

      外觀(Physical Appearance

      Liquid.

       

      儲存條件

       

      -85~-65℃保存,有效期2年。

       

      注意事項

       

      1.為了您的安全和健康,請穿實驗服并戴一次性手套操作。

      2.本產(chǎn)品僅作科研用途。

       

      產(chǎn)品數(shù)據(jù)

       

      SDS-PAGE

      Human FGF basic/FGF2/bFGF on SDS-PAGE under reduced condition.

      The purity is greater than 95%.

      SEC-HPLC

       The purity of Human bFGF is greater than 95% as determined by SEC-HPLC.

      Cell Based Assay

      The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is

      less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.

       

       

      Ver. CN20241030

       

      400-6111-883