產(chǎn)品描述
白介素10(IL-10)是一種免疫抑制細(xì)胞因子,由多種哺乳動物細(xì)胞類型產(chǎn)生,包括巨噬細(xì)胞、單核細(xì)胞、T細(xì)胞、B細(xì)胞和角質(zhì)形成細(xì)胞。IL-10抑制促炎細(xì)胞因子如IL-1和TNF-α的表達(dá)。與IL-4一樣,IL-10增強(qiáng)體液免疫反應(yīng)并減弱細(xì)胞介導(dǎo)的免疫反應(yīng)。
本品重組人IL-10以凍干粉形式提供,高活性、高純度、低內(nèi)毒素。
性能參數(shù)
別名 |
Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451 |
表達(dá)系統(tǒng) |
HEK293 |
表達(dá)區(qū)間 |
Ser19-Asn178 |
分子量 |
The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
內(nèi)毒素 |
< 1.0 EU/μg by the LAL method. |
純度 |
> 95% ,Tris-Bis PAGE和 HPLC檢測 |
生物活性 |
【ELISA Data】Immobilized Human IL-10, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, His Tag with the EC50 of 28.7ng/ml determined by ELISA. 【SPR Data】Human IL-10R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay. |
制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外觀 |
白色凍干粉 |
復(fù)溶方法 |
開蓋之前,先離心。使用去離子水復(fù)溶至濃度不低于0.1 mg/mL。建議首次使用時將蛋白分裝為每次實驗用量,避免反復(fù)凍融。 |
儲存條件
-25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, -85~-65℃保存,3個月有效期。
注意事項
Ver. CN20250415