Q:有沒有細胞特異性？會不會 293 細胞不好染， MSC 好染？

A:沒有。這些染料是親脂性染料，是通過插入到脂質(zhì)膜中進而被激光激發(fā)觀察，對于動植物細胞沒有特異性 。

Q:我用了無菌的 PBS 稀釋 DiI，發(fā)現(xiàn)稀釋的溶液中含有顆粒狀？

A:可以將稀釋液加熱或超聲一段時間，或者加入少量的DMSO 促溶。

Q:可以染細菌細胞膜嗎？

A:理論上可以，細菌有一層細胞壁，要先去除細胞壁，再染色。

Q:植物細胞和組織適用嗎？

A:理論上可以，一般植物細胞或者組織要制成原生質(zhì)體。

Q:可以染外泌體嗎？

A:外泌體具備膜結構，理論上可以被染色，但未測試過染色效果，需參考文獻。

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