產(chǎn)品說明書
FAQ
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已發(fā)表文獻
Hifair® Ⅲ 1st Strand cDNA Synthesis SuperMix基于Hifair® Ⅲ Reverse Transcriptase而開發(fā)的即用型預(yù)混液。與Hifair® Ⅱ Reverse Transcriptase相比，Hifair® Ⅲ Reverse Transcriptase熱穩(wěn)定性大幅度提高，可耐受高達60℃的反應(yīng)溫度，適合具有復(fù)雜二級結(jié)構(gòu)的RNA模板的逆轉(zhuǎn)錄。同時，該酶增強了與模板的親和力，非常適合少量模板以及低拷貝基因的逆轉(zhuǎn)錄。
Hifair® Ⅲ 1st Strand cDNA Synthesis SuperMix為即用型預(yù)混液，包含Hifair® Ⅲ Reverse Transcriptase，RNase inhibitor，dNTP，Random primers/Oligo dT primer mix和優(yōu)化的緩沖體系，只需再加入模板RNA和RNase-free ddH2O即可進行反應(yīng)。
產(chǎn)品組分
|
組分編號 |
組分名稱 |
產(chǎn)品編號/規(guī)格 |
|
|
11137ES10 (10 T) |
11137ES60 (100 T) |
||
|
11137-A |
RNase-free H2O |
1 mL |
2×1 mL |
|
11137-B |
4×Hifair® Ⅲ SuperMix |
50 μL |
500 μL |
產(chǎn)品應(yīng)用
適用于 RT-qPCR 實驗。
運輸和保存方法
冰袋運輸。-20ºC保存，有效期18個月。
第一鏈 cDNA 合成操作步驟
|
逆轉(zhuǎn)錄反應(yīng)體系 |
逆轉(zhuǎn)錄程序 |
||
|
組分 |
使用量 |
溫度 |
時間 |
|
RNase-free H2O |
To 20 μL |
25℃ |
5 min |
|
4×Hifair® Ⅲ SuperMix |
5 μL |
55℃ |
15 min |
|
Total RNA |
10 pg -5 μg |
85℃ |
5 min |
|
or mRNA |
10 pg-500 ng |
||
【注】：1. 20 μL逆轉(zhuǎn)錄反應(yīng)體系建議Total RNA的投入量不超過1 μg。如果目的基因的表達豐度低，最多投入5 μg Total RNA；
2. 逆轉(zhuǎn)錄溫度：推薦使用55℃。對于高GC含量模板或者復(fù)雜模板，可將逆轉(zhuǎn)錄溫度提高到60℃。
※ 逆轉(zhuǎn)錄產(chǎn)物可立即用于后續(xù)qPCR反應(yīng)，也可-20℃短期保存，若需長期保存，建議分裝后，于-80℃保存，避免反復(fù)凍融。
注意事項
1．可以室溫溶解，溶解后宜存放于冰盒內(nèi)或冰浴上，使用完畢后應(yīng)立即置于-20℃保存。
2．為了您的安全和健康，請穿實驗服并戴一次性手套操作。
3．本產(chǎn)品僅作科研用途！
HB220308
Q：逆轉(zhuǎn)錄試劑 11137ES 中引物是隨機引物還是oligo dT，還是二者的混合物？
A：二者的混合物。
Q：能否用來做miRNA/circRNA/lncRNA 的逆轉(zhuǎn)錄？
A：有A 尾的 lncRNA 可以逆轉(zhuǎn)錄， circRNA miRNA 和無 A 尾的 lncRNA 用 不能進行逆轉(zhuǎn)錄。microRNA 需要特殊的莖環(huán)引物， 需特別針對的逆轉(zhuǎn)錄試劑盒。
Q：11137 可以逆轉(zhuǎn)真菌（或其他物種）RNA 嗎？
A：RNA 的物種與逆轉(zhuǎn)錄沒有很大關(guān)系，RNA 質(zhì)量與逆轉(zhuǎn)錄有關(guān)。所以，得到 RNA 后，逆轉(zhuǎn)錄試劑盒都可以用，沒有特別針對的。
[1] Hu X, Pang J, Zhang J, et al. Discovery of Novel GR Ligands toward Druggable GR Antagonist Conformations Identified by MD Simulations and Markov State Model Analysis. Adv Sci (Weinh). 2022;9(3):e2102435. doi:10.1002/advs.202102435(IF:16.806)
[2] Zhong Z, Wu X, Wang Y, et al. Zn/Sr dual ions-collagen co-assembly hydroxyapatite enhances bone regeneration through procedural osteo-immunomodulation and osteogenesis. Bioact Mater. 2021;10:195-206. Published 2021 Sep 16. doi:10.1016/j.bioactmat.2021.09.013(IF:14.593)
[3] Liu J, Qu M, Wang C, et al. A Dual-Cross-Linked Hydrogel Patch for Promoting Diabetic Wound Healing. Small. 2022;18(17):e2106172. doi:10.1002/smll.202106172(IF:13.281)
[4] Cai Z, Zhang Y, Zhang W, et al. Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biol. 2022;52:102321. doi:10.1016/j.redox.2022.102321(IF:11.799)
[5] Yuan H, Zhao L, Yuan Y, et al. HBx represses WDR77 to enhance HBV replication by DDB1-mediated WDR77 degradation in the liver. Theranostics. 2021;11(17):8362-8378. Published 2021 Jul 25. doi:10.7150/thno.57531(IF:11.556)
[6] Kong D, Pan X, Jing Y, et al. ZmSPL10/14/26 are required for epidermal hair cell fate specification on maize leaf. New Phytol. 2021;230(4):1533-1549. doi:10.1111/nph.17293(IF:10.152)
[7] Mao S, Wang B, Yue L, Xia W. Effects of citronellol grafted chitosan oligosaccharide derivatives on regulating anti-inflammatory activity. Carbohydr Polym. 2021;262:117972. doi:10.1016/j.carbpol.2021.117972(IF:9.381)
[8] Wang S, Li Y, Zhong L, et al. Efficient gene editing through an intronic selection marker in cells. Cell Mol Life Sci. 2022;79(2):111. Published 2022 Jan 31. doi:10.1007/s00018-022-04152-1(IF:9.261)
[9] Wang X, Chen M, Fang L. hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3. Mol Ther Nucleic Acids. 2021;26:122-134. Published 2021 Jul 16. doi:10.1016/j.omtn.2021.07.003(IF:8.886)
[10] Wang X, Hu Y, He W, et al. Whole-genome resequencing of the wheat A subgenome progenitor Triticum urartu provides insights into its demographic history and geographic adaptation [published online ahead of print, 2022 Jun 1]. Plant Commun. 2022;100345. doi:10.1016/j.xplc.2022.100345(IF:8.625)
[11] Deng X, Ye D, Hua K, et al. MIR22HG inhibits breast cancer progression by stabilizing LATS2 tumor suppressor. Cell Death Dis. 2021;12(9):810. Published 2021 Aug 26. doi:10.1038/s41419-021-04105-9(IF:8.469)
[12] Wang Y, Xue T, Wang M, et al. CRISPR-Cas13a cascade-based viral RNA assay for detecting SARS-CoV-2 and its mutations in clinical samples. Sens Actuators B Chem. 2022;362:131765. doi:10.1016/j.snb.2022.131765(IF:7.460)
[13] Fu W, Zhang M, Liao J, et al. Discovery of a Novel Androgen Receptor Antagonist Manifesting Evidence to Disrupt the Dimerization of the Ligand-Binding Domain via Attenuating the Hydrogen-Bonding Network Between the Two Monomers. J Med Chem. 2021;64(23):17221-17238. doi:10.1021/acs.jmedchem.1c01287(IF:7.446)
[14] Cen Y, Zou X, Zhong Q, et al. The TIAR-mediated Nrf2 response to oxidative stress is mediated through the Nrf2 noncoding 3'untranslated region in Spodoptera litura. Free Radic Biol Med. 2022;184:17-29. doi:10.1016/j.freeradbiomed.2022.03.016(IF:7.376)
[15] Shi W, Ma Q, Yin W, et al. StTINY3 enhances cold-induced sweetening resistance by coordinating starch resynthesis and sucrose hydrolysis in potato [published online ahead of print, 2022 May 2]. J Exp Bot. 2022;erac171. doi:10.1093/jxb/erac171(IF:6.992)
[16] Zhang DW, Ye JJ, Sun Y, et al. CD19 and POU2AF1 are Potential Immune-Related Biomarkers Involved in the Emphysema of COPD: On Multiple Microarray Analysis. J Inflamm Res. 2022;15:2491-2507. Published 2022 Apr 20. doi:10.2147/JIR.S355764(IF:6.922)
[17] Wang S, Huang J, Liu F, et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021;14:7107-7130. Published 2021 Dec 20. doi:10.2147/JIR.S344990(IF:6.922)
[18] Ma C, Zhang L, He T, et al. Single cell Raman spectroscopy to identify different stages of proliferating human hepatocytes for cell therapy. Stem Cell Res Ther. 2021;12(1):555. Published 2021 Oct 30. doi:10.1186/s13287-021-02619-9(IF:6.832)
[19] Jiang M, Tang T, Liang X, et al. Maternal sevoflurane exposure induces temporary defects in interkinetic nuclear migration of radial glial progenitors in the fetal cerebral cortex through the Notch signalling pathway. Cell Prolif. 2021;54(6):e13042. doi:10.1111/cpr.13042(IF:6.831)
[20] Zhang Y, Li Y, Sun C, et al. Effect of Pterostilbene, a Natural Derivative of Resveratrol, in the Treatment of Colorectal Cancer through Top1/Tdp1-Mediated DNA Repair Pathway. Cancers (Basel). 2021;13(16):4002. Published 2021 Aug 9. doi:10.3390/cancers13164002(IF:6.639)
[21] Wang S, Huang J, Tan KS, Deng L, Liu F, Tan W. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway. Oxid Med Cell Longev. 2022;2022:4636618. Published 2022 Jan 27. doi:10.1155/2022/4636618(IF:6.543)
[22] Hu X, Pang J, Chen C, et al. Discovery of novel non-steroidal selective glucocorticoid receptor modulators by structure- and IGN-based virtual screening, structural optimization, and biological evaluation. Eur J Med Chem. 2022;237:114382. doi:10.1016/j.ejmech.2022.114382(IF:6.514)
[23] Jiao C, Zou J, Chen Z, et al. Dietary Glutamine Inclusion Regulates Immune and Antioxidant System, as Well as Programmed Cell Death in Fish to Protect against Flavobacterium columnare Infection. Antioxidants (Basel). 2021;11(1):44. Published 2021 Dec 26. doi:10.3390/antiox11010044(IF:6.313)
[24] Ma D, Wei J, Chen S, et al. Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression. Front Oncol. 2021;11:653902. Published 2021 Apr 13. doi:10.3389/fonc.2021.653902(IF:6.244)
[25] Zhao LN, Yuan HF, Wang YF, et al. IFN-α inhibits HBV transcription and replication by promoting HDAC3-mediated de-2-hydroxyisobutyrylation of histone H4K8 on HBV cccDNA minichromosome in liver. Acta Pharmacol Sin. 2022;43(6):1484-1494. doi:10.1038/s41401-021-00765-7(IF:6.150)
[26] Yuan HF, Zhao M, Zhao LN, et al. PRMT5 confers lipid metabolism reprogramming, tumour growth and metastasis depending on the SIRT7-mediated desuccinylation of PRMT5 K387 in tumours [published online ahead of print, 2022 Jan 19]. Acta Pharmacol Sin. 2022;10.1038/s41401-021-00841-y. doi:10.1038/s41401-021-00841-y(IF:6.150)
[27] Pang JP, Shen C, Zhou WF, et al. Discovery of novel antagonists targeting the DNA binding domain of androgen receptor by integrated docking-based virtual screening and bioassays. Acta Pharmacol Sin. 2022;43(1):229-239. doi:10.1038/s41401-021-00632-5(IF:6.150)
[28] Pang JP, Hu XP, Wang YX, et al. Discovery of a novel nonsteroidal selective glucocorticoid receptor modulator by virtual screening and bioassays [published online ahead of print, 2022 Feb 2]. Acta Pharmacol Sin. 2022;1-10. doi:10.1038/s41401-021-00855-6(IF:6.150)
[29] Zi Z, Zhao H, Wang H, Ma X, Wei F. B7-H3 Chimeric Antigen Receptor Redirected T Cells Target Anaplastic Lymphoma Kinase-Positive Anaplastic Large Cell Lymphoma. Cancers (Basel). 2020;12(12):3815. Published 2020 Dec 17. doi:10.3390/cancers12123815(IF:6.126)
[30] Yu X, Hu L, Li S, et al. Long non-coding RNA Taurine upregulated gene 1 promotes osteosarcoma cell metastasis by mediating HIF-1α via miR-143-5p. Cell Death Dis. 2019;10(4):280. Published 2019 Mar 25. doi:10.1038/s41419-019-1509-1(IF:5.959)
[31] Shi X, Ye C, Qin X, et al. Novel Pituitary Actions of TAC4 Gene Products in Teleost. Int J Mol Sci. 2021;22(23):12893. Published 2021 Nov 29. doi:10.3390/ijms222312893(IF:5.924)
[32] Yang N, Zhang X, Li L, et al. Ginsenoside Rc Promotes Bone Formation in Ovariectomy-Induced Osteoporosis In Vivo and Osteogenic Differentiation In Vitro. Int J Mol Sci. 2022;23(11):6187. Published 2022 May 31. doi:10.3390/ijms23116187(IF:5.924)
[33] Wang X, Li W, Jiang H, et al. Zebrafish Xenograft Model for Studying Pancreatic Cancer-Instructed Innate Immune Microenvironment. Int J Mol Sci. 2022;23(12):6442. Published 2022 Jun 9. doi:10.3390/ijms23126442(IF:5.924)
[34] Jiang R, Xu J, Zhang Y, Zhu X, Liu J, Tan Y. Ligustrazine Alleviate Acute Lung Injury Through Suppressing Pyroptosis and Apoptosis of Alveolar Macrophages. Front Pharmacol. 2021;12:680512. Published 2021 May 28. doi:10.3389/fphar.2021.680512(IF:5.811)
[35] Xie T, Chen X, Chen W, et al. Curcumin is a Potential Adjuvant to Alleviates Diabetic Retinal Injury via Reducing Oxidative Stress and Maintaining Nrf2 Pathway Homeostasis. Front Pharmacol. 2021;12:796565. Published 2021 Dec 10. doi:10.3389/fphar.2021.796565(IF:5.811)
[36] Sun L, Cao Y, Kong Q, et al. Over-expression of the bottlenose dolphin Hoxd13 gene in zebrafish provides new insights into the cetacean flipper formation. Genomics. 2021;113(5):2925-2933. doi:10.1016/j.ygeno.2021.06.028(IF:5.736)
[37] Xia C, Qin X, Zhou L, et al. Reproductive Regulation of PrRPs in Teleost: The Link Between Feeding and Reproduction. Front Endocrinol (Lausanne). 2021;12:762826. Published 2021 Nov 3. doi:10.3389/fendo.2021.762826(IF:5.555)
[38] Yuan J, Wang JM, Li ZW, et al. Full-length transcriptome analysis reveals the mechanism of acupuncture at PC6 improves cardiac function in myocardial ischemia model. Chin Med. 2021;16(1):55. Published 2021 Jul 8. doi:10.1186/s13020-021-00465-8(IF:5.455)
[39] Wei MP, Yu H, Guo YH, Cheng YL, Xie YF, Yao WR. Synergistic combination of Sapindoside A and B: A novel antibiofilm agent against Cutibacterium acnes. Microbiol Res. 2022;254:126912. doi:10.1016/j.micres.2021.126912(IF:5.415)
[40] Zhang X, Zhang B, Li L, Li X, Zhang J, Chen G. Fermented noni (Morinda citrifolia L.) fruit juice improved oxidative stress and insulin resistance under the synergistic effect of Nrf2/ARE pathway and gut flora in db/db mice and HepG2 cells [published online ahead of print, 2022 Jul 14]. Food Funct. 2022;10.1039/d2fo00595f. doi:10.1039/d2fo00595f(IF:5.396)
[41] Yu S, Liu S, Wang N, et al. Novel insights into antidepressant mechanism of Kai Xin San formula: Inhibiting NLRP3 inflammasome activation by promoting autophagy. Phytomedicine. 2021;93:153792. doi:10.1016/j.phymed.2021.153792(IF:5.340)
[42] Wang X, Song H, Fang L, Wu T. EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway. Cell Death Discov. 2022;8(1):92. Published 2022 Mar 2. doi:10.1038/s41420-022-00892-y(IF:5.241)
[43] Qi M, Zhu X, Yu X, et al. Preparation of W/O Hypaphorine-Chitosan Nanoparticles and Its Application on Promoting Chronic Wound Healing via Alleviating Inflammation Block. Nanomaterials (Basel). 2021;11(11):2830. Published 2021 Oct 25. doi:10.3390/nano11112830(IF:5.076)
[44] Zhang Y, Sun M, Jian S, et al. mPEG2k-PCLx Polymeric Micelles Influence Pharmacokinetics and Hypoglycemic Efficacy of Metformin through Inhibition of Organic Cation Transporters in Rats. Mol Pharm. 2021;18(7):2586-2599. doi:10.1021/acs.molpharmaceut.1c00078(IF:4.939)
[45] Hu R, Zhu X, Chen C, Xu R, Li Y, Xu W. RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell Prolif. 2018;51(6):e12508. doi:10.1111/cpr.12508(IF:4.936)
[46] Li Z, Wang Y, Hu R, Xu R, Xu W. LncRNA B4GALT1-AS1 recruits HuR to promote osteosarcoma cells stemness and migration via enhancing YAP transcriptional activity. Cell Prolif. 2018;51(6):e12504. doi:10.1111/cpr.12504(IF:4.936)
[47] Wang Y, Hu W, Deng Z, He X. Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay. Appl Microbiol Biotechnol. 2021;105(7):2901-2909. doi:10.1007/s00253-021-11229-7(IF:4.813)
[48] Sun J, Ha N, Liu Z, Bian Q, Wang X. A Neural Crest-specific Overexpression Mouse Model Reveals the Transcriptional Regulatory Effects of Dlx2 During Maxillary Process Development. Front Physiol. 2022;13:855959. Published 2022 Apr 21. doi:10.3389/fphys.2022.855959(IF:4.566)
[49] Xu X, Liu W, Liu X, et al. Genetic manipulation of bermudagrass photosynthetic biosynthesis using Agrobacterium-mediated transformation. Physiol Plant. 2022;174(3):e13710. doi:10.1111/ppl.13710(IF:4.500)
[50] Wang Y, Zhu GQ, Tian D, et al. Comprehensive analysis of tumor immune microenvironment and prognosis of m6A-related lncRNAs in gastric cancer. BMC Cancer. 2022;22(1):316. Published 2022 Mar 24. doi:10.1186/s12885-022-09377-8(IF:4.430)
[51] Yue L, Lin H, Yuan S, et al. miR-1251-5p Overexpression Inhibits Proliferation, Migration, and Immune Escape in Clear Cell Renal Cell Carcinoma by Targeting NPTX2. J Oncol. 2022;2022:3058588. Published 2022 Mar 10. doi:10.1155/2022/3058588(IF:4.375)
[52] Wang Y, Zhang J, Xu Z, et al. Identification and action mechanism of lipid regulating components from Rhei Radix et rhizoma. J Ethnopharmacol. 2022;292:115179. doi:10.1016/j.jep.2022.115179(IF:4.360)
[53] Xiang B, Yang J, Zhang J, et al. The role of genes affected by human evolution marker GNA13 in schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry. 2020;98:109764. doi:10.1016/j.pnpbp.2019.109764(IF:4.315)
[54] Lei J, Zhang X, Tan R, Li Y, Zhao K, Niu H. Levels of lncRNA GAS5 in Plasma of Patients with Severe Traumatic Brain Injury: Correlation with Systemic Inflammation and Early Outcome. J Clin Med. 2022;11(12):3319. Published 2022 Jun 9. doi:10.3390/jcm11123319(IF:4.242)
[55] Ye W, Yao M, Dong Y, et al. Remdesivir (GS-5734) Impedes Enterovirus Replication Through Viral RNA Synthesis Inhibition [published correction appears in Front Microbiol. 2020 Nov 23;11:621197]. Front Microbiol. 2020;11:1105. Published 2020 Jun 12. doi:10.3389/fmicb.2020.01105(IF:4.236)
[56] Zhang Q, Wu JF, Shi QL, et al. The Neuronal Activation of Deep Cerebellar Nuclei Is Essential for Environmental Enrichment-Induced Post-Stroke Motor Recovery. Aging Dis. 2019;10(3):530-543. Published 2019 Jun 1. doi:10.14336/AD.2018.1220(IF:4.232)
[57] Lv N, Zhao M, Han Y, et al. The roles of bone morphogenetic protein 2 in perfluorooctanoic acid induced developmental cardiotoxicity and l-carnitine mediated protection. Toxicol Appl Pharmacol. 2018;352:68-76. doi:10.1016/j.taap.2018.05.028(IF:4.219)
[58] Su H, Liang J, Abou-Elwafa SF, et al. ZmCCT regulates photoperiod-dependent flowering and response to stresses in maize. BMC Plant Biol. 2021;21(1):453. Published 2021 Oct 6. doi:10.1186/s12870-021-03231-y(IF:4.215)
[59] Shan N, Zhang Y, Xu Y, et al. Ethylene-induced potassium transporter AcKUP2 gene is involved in kiwifruit postharvest ripening. BMC Plant Biol. 2022;22(1):108. Published 2022 Mar 9. doi:10.1186/s12870-022-03498-9(IF:4.215)
[60] Hu Q, Xu S, Ye C, Jia J, Zhou L, Hu G. Novel Pituitary Actions of Epidermal Growth Factor: Receptor Specificity and Signal Transduction for UTS1, EGR1, and MMP13 Regulation by EGF. Int J Mol Sci. 2019;20(20):5172. Published 2019 Oct 18. doi:10.3390/ijms20205172(IF:4.183)
[61] Cui P, Lin Q, Fang D, et al. Tung Tree (Vernicia fordii, Hemsl.) Genome and Transcriptome Sequencing Reveals Co-Ordinate Up-Regulation of Fatty Acid β-Oxidation and Triacylglycerol Biosynthesis Pathways During Eleostearic Acid Accumulation in Seeds. Plant Cell Physiol. 2018;59(10):1990-2003. doi:10.1093/pcp/pcy117(IF:4.059)
[62] Wang Y, Li N, Tian D, et al. Analysis of m6A-Related lncRNAs for Prognosis Value and Response to Immune Checkpoint Inhibitors Therapy in Hepatocellular Carcinoma. Cancer Manag Res. 2021;13:6451-6471. Published 2021 Aug 16. doi:10.2147/CMAR.S322179(IF:3.989)
[63] Xue J, Chen L, Cheng H, et al. The Identification and Validation of Hub Genes Associated with Acute Myocardial Infarction Using Weighted Gene Co-Expression Network Analysis. J Cardiovasc Dev Dis. 2022;9(1):30. Published 2022 Jan 17. doi:10.3390/jcdd9010030(IF:3.948)
[64] Hou J, Sun Y, Wang L, Jiang Y, Chen N, Tong S. Genome-Wide Analysis of the Homeobox Gene Family and Identification of Drought-Responsive Members in Populus trichocarpa. Plants (Basel). 2021;10(11):2284. Published 2021 Oct 25. doi:10.3390/plants10112284(IF:3.935)
[65] Wu J, Lu J, Huang J, et al. Variations in Energy Metabolism Precede Alterations in Cardiac Structure and Function in Hypertrophic Preconditioning. Front Cardiovasc Med. 2020;7:602100. Published 2020 Dec 11. doi:10.3389/fcvm.2020.602100(IF:3.915)
[66] Guo Q, Li Y, Chen Y, et al. β-Elemene induces apoptosis by activating the P53 pathway in human hypertrophic scar fibroblasts. IUBMB Life. 2022;74(6):508-518. doi:10.1002/iub.2614(IF:3.885)
[67] Jiang SJ, Xiao X, Zheng J, et al. Antibacterial and antibiofilm activities of novel antimicrobial peptide DP7 against the periodontal pathogen Porphyromonas gingivalis [published online ahead of print, 2022 May 14]. J Appl Microbiol. 2022;10.1111/jam.15614. doi:10.1111/jam.15614(IF:3.772)
[68] Zhang F, Teng Z, Wang L, Wang L, Huang T, Zhang X. Dietary Selenium Deficiency and Excess Accelerate Ubiquitin-Mediated Protein Degradation in the Muscle of Rainbow Trout (Oncorhynchus mykiss) via Akt/FoxO3a and NF-κB Signaling Pathways. Biol Trace Elem Res. 2022;200(3):1361-1375. doi:10.1007/s12011-021-02726-x(IF:3.738)
[69] YujiaLiu, Shi C, Zhang G, et al. Antimicrobial mechanism of 4-hydroxyphenylacetic acid on Listeria monocytogenes membrane and virulence. Biochem Biophys Res Commun. 2021;572:145-150. doi:10.1016/j.bbrc.2021.07.096(IF:3.575)
[70] Fu H, Zhang Z, Dai Y, Liu S, Fu E. Brequinar inhibits enterovirus replication by targeting biosynthesis pathway of pyrimidines. Am J Transl Res. 2020;12(12):8247-8255. Published 2020 Dec 25. (IF:3.375)
[71] Shen E, Zhang J, Lu Y. DEP domain containing 1B (DEPDC1B) exerts the tumor promoter in hepatocellular carcinoma through activating p53 signaling pathway via kinesin family member 23 (KIF23). Bioengineered. 2022;13(1):1103-1114. doi:10.1080/21655979.2021.2017629(IF:3.269)
[72] Zhang Y, Zhang R, Lu L, et al. Knockdown of lectin-like oxidized low-density lipoprotein-1 ameliorates alcoholic cardiomyopathy via inactivating the p38 mitogen-activated protein kinase pathway. Bioengineered. 2022;13(4):8926-8936. doi:10.1080/21655979.2022.2056814(IF:3.269)
[73] Mei X, Din H, Zhao J, Tong J, Zhu W. Transcription factor Krüppel-like factor 5-regulated N-myc downstream-regulated gene 2 reduces IL-1β-induced chondrocyte inflammatory injury and extracellular matrix degradation. Bioengineered. 2021;12(1):7020-7032. doi:10.1080/21655979.2021.1971483(IF:3.269)
[74] Cao S, Li X, Feng T, et al. Hirudin promotes proliferation and osteogenic differentiation of HBMSCs via activation of cyclic guanosine monophosphate (cGMP)/protein kinase-G (PKG) signaling pathway. Bioengineered. 2022;13(3):6061-6069. doi:10.1080/21655979.2021.2008697(IF:3.269)
[75] Xu S, Zhou L, Guo S, et al. Different pituitary action of NK3Ra and NK3Rb in grass carp. Gen Comp Endocrinol. 2021;313:113829. doi:10.1016/j.ygcen.2021.113829(IF:2.822)
[76] Wang Y, Zhao S, Li G, Wang D, Jin Y. Neuroprotective Effect of HOTAIR Silencing on Isoflurane-Induced Cognitive Dysfunction via Sponging microRNA-129-5p and Inhibiting Neuroinflammation [published online ahead of print, 2022 Jan 13]. Neuroimmunomodulation. 2022;1-11. doi:10.1159/000521014(IF:2.492)
[77] Li J, Xu S. Tilianin attenuates MPP+-induced oxidative stress and apoptosis of dopaminergic neurons in a cellular model of Parkinson's disease. Exp Ther Med. 2022;23(4):293. doi:10.3892/etm.2022.11223(IF:2.447)
[78] He L, Fan X, Li Y, et al. Aberrant methylation status of SPG20 promoter in hepatocellular carcinoma: A potential tumor metastasis biomarker. Cancer Genet. 2019;233-234:48-55. doi:10.1016/j.cancergen.2019.04.003(IF:2.183)
[79] Qu D, Tan XH, Zhang KK, Wang Q, Wang HJ. ATF3 mRNA, but not BTG2, as a possible marker for vital reaction of skin contusion. Forensic Sci Int. 2019;303:109937. doi:10.1016/j.forsciint.2019.109937(IF:1.990)
