RPMI 1640培養(yǎng)基以研發(fā)地點(diǎn)羅斯韋爾公園紀(jì)念研究所(Roswell Park Memorial Institute. RPMI)命名,1640為培養(yǎng)基代號(hào)。它是McCoy's 5A培養(yǎng)基的改進(jìn)型,使用碳酸氫鹽緩沖系統(tǒng)。RPMI 1640培養(yǎng)基最初開發(fā)用于人白血病細(xì)胞的懸浮或單層培養(yǎng),后來被發(fā)現(xiàn)也適用于多種哺乳動(dòng)物細(xì)胞,包括HeLa、Jurkat、MCF-7、PC-12、PBMC、星形膠質(zhì)細(xì)胞和癌細(xì)胞,尤其適用于懸浮細(xì)胞的培養(yǎng),是使用最為廣泛的培養(yǎng)基之一。
RPMI 1640培養(yǎng)基與其它培養(yǎng)基的區(qū)別在于含有還原型谷胱甘肽和高濃度的維生素。RPMI 1640培養(yǎng)基含有EMEM和DMEM中沒有的生物素、維生素B12和對(duì)氨基苯甲酸,以及高濃度的氯化膽堿和肌醇。
本產(chǎn)品含有:D-葡萄糖、酚紅、L-谷氨酰胺。
不含有:丙酮酸鈉、HEPES。
產(chǎn)品成分
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
Amino Acids |
|||
Glycine |
75.0 |
10.0 |
0.13333334 |
L-Arginine |
174.0 |
200.0 |
1.1494253 |
L-Asparagine |
132.0 |
50.0 |
0.37878788 |
L-Aspartic acid |
133.0 |
20.0 |
0.15037593 |
L-Cystine 2HCl |
313.0 |
65.0 |
0.20766774 |
L-Glutamic Acid |
147.0 |
20.0 |
0.13605443 |
L-Glutamine |
146.0 |
300.0 |
2.0547945 |
L-Histidine |
155.0 |
15.0 |
0.09677419 |
L-Hydroxyproline |
131.0 |
20.0 |
0.15267175 |
L-Isoleucine |
131.0 |
50.0 |
0.3816794 |
L-Leucine |
131.0 |
50.0 |
0.3816794 |
L-Lysine hydrochloride |
183.0 |
40.0 |
0.21857923 |
L-Methionine |
149.0 |
15.0 |
0.10067114 |
L-Phenylalanine |
165.0 |
15.0 |
0.09090909 |
L-Proline |
115.0 |
20.0 |
0.17391305 |
L-Serine |
105.0 |
30.0 |
0.2857143 |
L-Threonine |
119.0 |
20.0 |
0.16806723 |
L-Tryptophan |
204.0 |
5.0 |
0.024509804 |
L-Tyrosine disodium salt dihydrate |
261.0 |
29.0 |
0.11111111 |
L-Valine |
117.0 |
20.0 |
0.17094018 |
Vitamins |
|||
Biotin |
244.0 |
0.2 |
8.1967213E-4 |
Choline chloride |
140.0 |
3.0 |
0.021428572 |
D-Calcium pantothenate |
477.0 |
0.25 |
5.24109E-4 |
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
Niacinamide |
122.0 |
1.0 |
0.008196721 |
Para-Aminobenzoic Acid |
137.0 |
1.0 |
0.00729927 |
Pyridoxine hydrochloride |
206.0 |
1.0 |
0.004854369 |
Riboflavin |
376.0 |
0.2 |
5.319149E-4 |
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
Vitamin B12 |
1355.0 |
0.005 |
3.690037E-6 |
i-Inositol |
180.0 |
35.0 |
0.19444445 |
Inorganic Salts |
|||
Calcium nitrate (Ca(NO3)2 4H2O) |
236.0 |
100.0 |
0.42372882 |
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
48.84 |
0.407 |
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
Sodium Chloride (NaCl) |
58.0 |
6000.0 |
103.44827 |
Sodium Phosphate dibasic (Na2HPO4-7H2O) |
268.0 |
800.0 |
2.9850745 |
Sodium Bicarbonate (NaHCO3) |
84.0 |
2000.0 |
23.8 |
Other Components |
|||
D-Glucose (Dextrose) |
180.0 |
2000.0 |
11.111111 |
Glutathione (reduced) |
307.0 |
1.0 |
0.0032573289 |
Phenol Red |
376.4 |
5.0 |
0.013283741 |
運(yùn)輸和保存方法
2-8℃避光保存,一年有效。
注意事項(xiàng)
1)為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
2)本產(chǎn)品僅作科研用途。
HB211229