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      Recombinant Mouse IL-1α
       
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      Product Information
      


      
	
		
      
			
			
      Product Name
      
			      		
			
			
      Cat#
      
			      		
			
			
      Size
      
			      		
		
      
		
      
			
			
      Recombinant Mouse IL-1α Protein
      
			      		
			
			
      90139ES05
      
			      		
			
			
      2 μg
      
			      		
		
      
		
      
			
			
      90139ES10
      
			      		
			
			
      10 μg
      
			      		
		
      
		
      
			
			
      90139ES50
      
			      		
			
			
      50 μg
      
			      		
		
      
		
      
			
			
      90139ES60
      
			      		
			
			
      100 μg
      
			      		
		
      
		
      
			
			
      90139ES76
      
			      		
			
			
      500 μg
      
			      		
		
      
	      

      


       
      


      Product Description
      


      Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1,and is a cytokine of the interleukin-1 family. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. IL-1 is a major immunoregulatory/proinflammatory cytokine which also affects fibroblast proliferation and function and therefore it was of interest to investigate whether its constitutive expression influences the in vivo tumorigenic potential of transformed fibroblastoid cell lines.
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      Interleukin-1 alpha, IL-1α, BAF, IL-1F1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
       Q3U0Y6
      
			      		
		
      
		
      
			
			
      GeneID
      
			      		
			
			
      16175
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived mouse IL-1alpha protein, Ser115-Ala270.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 17.9 kDa.
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 97% by SDS-PAGE and HPLC analyses.
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 × 108 IU/mg. Fully biologically active when compared to standard.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. 
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      

      


       
      


        
	
      1. Avoid repeated freeze-thaw cycles.
        2. 
	
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
        3. 
	
      3. For research use only!
        4. 

      


      HB240321
      


       
      


       
      


       
      


       
      


       
      



      
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      HB220210
      

         
       
       
       
       
       
       
       
      400-6111-883