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      Recombinant Human TWEAK Receptor/TNFRSF12A
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻
                  
       
      TWEAKR belongs to the TNF family of transmembrane proteins, and contains a cytoplasmic "death domain", which can activate a cell's apoptotic machinery. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAKR can be activated by either membrane-anchored or soluble TWEAK. Recombinant Human soluble TWEAKR is a 53 amino acid polypeptide (5.6 kDa) comprising the entire extracellular domain of the full-length TWEAKR protein.
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      TNFRSF12A, FGF-inducible 14, CD266
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      Q9NP84
      
			      		
		
      
		
      
			
			
      GeneID
      
			      		
			
			
      51330
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived Human TWEAK Receptor/TNFRSF12A protein,Glu28-Pro80.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 5.6 kDa
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 95% by SDS-PAGE and HPLC analyses.
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/mL of rHuTWEAK. Fully biologically active when compared to standard. 
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      


      1. Avoid repeated freeze-thaw cycles.
      


      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      


      3. For research use only.
      


      HB220304
      


       
      

         
       
       
       
       
       
       
       
      400-6111-883