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      Recombinant HBsAg-preS1 Protein
       
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      產(chǎn)品說(shuō)明書(shū)
                  
      FAQ
                  
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      Product Description
      


       
      


      Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1.
      


       
      


      Product Properties
      


       
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      HBV Surface Antigen-preS1 ; L glycoprotein，L-HBsAg，Large S protein，Large surface protein，Major surface antigen.
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived HBsAg-preS1
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acids，with an N-terminal Met
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      MGGWSSKPRQ GMGTNLSVPN PLGFFPDHQL DPAFGANSNN PDWDFNPNKD HWPEAHQVGA GAFGPGFTPP HGGLLGWSPQ AQGILTTVPV APPPASTNRQ SGRQPTPISP PLRDSHPQA
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 97% by SDS-PAGE and HPLC analyses.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
      
			      		
		
      
		
      
			
			
      Applications
      
			      		
			
			
      1. Immunochromatography (capture and conjugate);
      
			2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS1;
      
			3. ELISA.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


       
      


      The products are shipped with ice pack and can be stored at -20℃ for 1 year.
      


      1 month, 2 to 8 °C under sterile conditions after reconstitution.
      
3 months, -20 °C under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      


       
      


        
	
      1. For your safety and health, please wear lab coats and disposable gloves for operation.   
        2. 
	
      2. For research use only.
        3. 

      


       
      


      HB20241104
      

         
       
       
       
       
       
       
       
      400-6111-883