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      Recombinant Human Epigen
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
      Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, and heart, as well as in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human Epigen is a 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF-homologous portion of the Epigen precursor.
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      EPG, Epithelial Mitogen
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      Q6UW88
      
			      		
		
      
		
      
			
			
      GeneID
      
			      		
			
			
      255324
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived Human Epigen protein,Ala24-Ala104.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 7.9 kDa.
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 98 % by SDS-PAGE and HPLC analyses.
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/mL, corresponding to a specific activity of > 3.3 × 103 IU/mg.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      


      1. Avoid repeated freeze-thaw cycles.
      


      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      


      3. For research use only.
      


      HB220318
      

         
       
       
       
       
       
       
       
      400-6111-883