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      Recombinant Human PlGF-2
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
      PlGF-2 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors.  PlGF-2 is expressed in umbilical vein endothelial cells and placenta.  It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and migration.  PlGF-2 also signals through Neuropilin (NP-1), and can bind with high affinity to heparin. Recombinant Human PlGF-2 is a 34.6 kDa disulfide-linked homodimeric protein of two 152 amino acid polypeptide chains.
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      Placenta Growth Factor-2, PGFL
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      P49763-3
      
			      		
		
      
		
      
			
			
      GeneID
      
			      		
			
			
      5228
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived Human PlGF-2 protein,Leu19-Arg131.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 34.6 kDa.
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 97% by SDS-PAGE and HPLC analyses.
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      Fully biologically active when compared to standard. The biologically active as determined by its ability to chemoattract human monocytes using a concentration range of 5.0-50 ng/mL.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 0.1 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      


      1. Avoid repeated freeze-thaw cycles.
      


      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      


      3. For research use only.
      


      HB220318
      


       
      

         
       
       
       
       
       
       
       
      400-6111-883