成人欧美一区二区三区,天天干天天操天天爱,免费特黄一级欧美大片在线看 ,亚洲中文字幕无码一区日日添

    <span id="alen4"></span>

    <li id="alen4"></li>
    

      
    
        
       
       
       
       
      Recombinant Human Ubiquitin Activating Enzyme E1(UBE1)
       
      更多活動(dòng)更多優(yōu)惠，盡在翌圣商城》
       
       
       
      批量采購， 請點(diǎn)擊詢價(jià)
       
       
      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
      產(chǎn)品簡介
      


       
      


      This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.
      


       
      


      產(chǎn)品性質(zhì)
      


       
      


      
	
		
      
			
			
      別名(Synonyms)
      
			      		
			
			
      UBA1
      
			      		
		
      
		
      
			
			
      來源(Source)
      
			      		
			
			
      E.coli
      
			      		
		
      
		
      
			
			
      分子量
      

			
      (Molecular Weight)
      
			      		
			
			
      Approximately 118 kDa.
      
			      		
		
      
		
      
			
			
      氨基酸序列
      

			
      (AA Sequence)
      
			      		
			
			
      MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT
      

			
      SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY
      

			
      DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP
      

			
      GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
      
			      		
		
      
		
      
			
			
      外觀
      
			(Physical Appearance)
      
			      		
			
			
      Liquid
      
			      		
		
      
		
      
			
			
      標(biāo)簽(Tag)
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      純度(Purity)
      
			      		
			
			
      > 95% by SDS-PAG
      
			      		
		
      
		
      
			
			
      緩沖液(Buffer)
      
			      		
			
			
      50 mM HEPES, pH8.0, 50mM NaCl.
      
			      		
		
      
	      

      


       
      


      儲(chǔ)存條件
      


       
      


      -25~-15℃保存
      


       
      


      注意事項(xiàng)
      


       
      


      1. 避免反復(fù)凍融。
      


      2. 本產(chǎn)品僅作科研用途。
      


      2. 為了您的安全和健康，請穿實(shí)驗(yàn)服并佩戴一次性手套操作。
      


       
      


      反應(yīng)緩沖液配方
      


      
	
		
      
			
			
      名稱
      
			      		
			
			
      組分
      
			      		
			
			
      濃度
      
			      		
		
      
		
      
			
			
       
      

			
      10 × Reaction Buffer（200 ul）
      
			      		
			
			
      Tris PH8.0
      
			      		
			
			
      500 mM
      
			      		
		
      
		
      
			
			
      DTT
      
			      		
			
			
      6 mM
      
			      		
		
      
		
      
			
			
      ddH2O 
      
			      		
			
			
      to 200μL
      
			      		
		
      
		
      
			
			
       
      

			
      10 x ATP-Mg （200 ul）
      

			
       
      
			      		
			
			
      ATP
      
			      		
			
			
      100 mM
      
			      		
		
      
		
      
			
			
      MgCl2
      
			      		
			
			
      100 mM
      
			      		
		
      
		
      
			
			
      ddH2O 
      
			      		
			
			
      to 200μL
      
			      		
		
      
	      

      


       
      


      參考陽性反應(yīng)體系
      


      
	
		
      
			
			
      組分
      
			      		
			
			
      體積 (μL)
      
			      		
		
      
		
      
			
			
      ddH2O 
      
			      		
			
			
      to 20 μL
      
			      		
		
      
		
      
			
			
      10 × Reaction Buffer 
      
			      		
			
			
      2 μL
      
			      		
		
      
		
      
			
			
      10 × E1 Enzyme
      
			      		
			
			
      2 μL
      
			      		
		
      
		
      
			
			
      5 × Ubiquitin
      
			      		
			
			
      4 μL
      
			      		
		
      
		
      
			
			
      10 × ATP-Mg
      
			      		
			
			
      2 μL
      
			      		
		
      
		
      
			
			
      20 × E2 Enzyme
      
			      		
			
			
      1 μL
      
			      		
		
      
		
      
			
			
      10 × E3 Ligase Itch (Positive control)
      
			      		
			
			
      2 μL
      
			      		
		
      
		
      
			
			
      20 × S5a substrate (Positive control)
      
			      		
			
			
      1 μL
      
			      		
		
      
	      

      


       
      


      Ver.CN20250428
      

         
       
       
       
       
       
       
       
      400-6111-883