This product is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Its recognition sequence is 5'-GCTCTTCN1/N4-3'. Use to digest plasmids to prepare poly(A/T/G/C)-terminated linearized DNA fragments to obtain specific cohesive ends.

This product is produced in accordance with GMP process requirements and provided in a liquid form.

 

Product Properties

Source	Recombinant E. coli with BspQI gene
Reaction   Temperature	50℃
Storage   Conditions	20 mM Tris-HCl, 0.5 M KCl, 0.1 mM EDTA, 1mM DTT, 0.1% Triton X-100, 50%   Glycerol
Unit   Definition	1 unit: The amount of enzyme required to   digest 1 μg of λDNA   within 1 h at 50℃ in a 50 μL system

 

Contents

Contents No.	Name	Catalog No./Specification
		10664ES76 (500 U)	10664ES86  (2500 U)	10664ES92 (10 KU)	10664ES98 (100KU)
10664	BspQI GMP-grade (10 U/μL)	50 μL	250 μL	1 mL	10 mL

 

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

 

Applications

1. Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);

2. Digestion of DNA to obtain specific sticky ends.

 

Experimental methods

50 μL reaction system

This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.

1）Add the following components in sequence:

Components	Volume
Plasmid DNA	1-2 μg
10×Digestion Buffer 3	5.0 μL
BspQI (10 U/μL)	1.0 μL
RNase-free ddH2O	Up to 50 μL

Note: 10× Digestion Buffer 3(Cat#10667): 500mM Tris-HCl, 1M NaCl,100mM MgCl₂, 1mg/mL OsrHSA, pH7.9@25℃

2）Incubate at 50°C 1 h;

3）DNA linearization is complete, and subsequent experiments can be performed.

 

Notes:

1. The volume of restriction endonuclease added should not exceed 1/10 of the reaction volume;

2. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.

HB220701