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      Recombinant Human MIP-4/CCL18 重組人MIP-4/CCL18

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

      Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell-mediated immunity responses.

       

      Product Properties

      Synonyms

      AMAC-1; AMAC1; CCL18; CKb7; DC-CK1; DCCK1; MIP-4; MIP4; PARC ; SCYA18

      Accession

      P55774

      GeneID

      6362

      Source

      E.coli-derived human Macrophage Inflammatory Protein-4, Ala21-Ala89

      Molecular Weight

      Approximately 7.9 kDa.

      AA Sequence

      AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI LLTKRGRQIC ADPNKKWVQK YISDLKLNA

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 97 % by SDS-PAGE and HPLC analyses.

      Biological Activity

      Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 1.0-10 ng/ml.

      Endotoxin

      <1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.

      2. For your safety and health, please wear lab coats and disposable gloves for operation.

      3. For research use only.

       

                                                                                                        HB220505

      400-6111-883