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      Recombinant Mouse RSPO1/R-Spondin-1 Protein重組小鼠RSPO1蛋白
       
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      R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids .
      


       
      


      性能參數(shù)
      


       
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      R-Spondin 1;Roof Plate-specific Spondin 1；Cristin3; R-spondin-1
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      Expressed in E. coli.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 20.9kDa, a single non-glycosylated polypeptide chain containing 189 amino acid
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      Q9Z132: Ser21-Gly209
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 96% by SDS-PAGE.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      ＜0.01EU/μg protein as determined by LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      Before use this product, please read the direction below carefully.
      

			
      1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.
      

			
      2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. 
      

			
      Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
      
			      		
		
      
	      

      


       
      


      儲(chǔ)存條件
      


       
      


      The product should be stored at -25~-15℃ for 1 year from date of receipt.
      


      1 month, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項(xiàng)
      


       
      


      1.Please operate with lab coats and disposable gloves,for your safety.
      


      2.This product is for research use only. 
      


                                              Ver. CN20231116
      

         
       
       
       
       
       
       
       
      400-6111-883