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      Recombinant Human TGF-alpha Protein
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
      Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines.  TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF
      
			      		
		
      
		
      
			
			
      Accession
      
			      		
			
			
      P01135
      
			      		
		
      
		
      
			
			
      Gene ID
      
			      		
			
			
      7039
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      E.coli-derived Human TGF-a protein, Vla40-Ala89 
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Approximately 5.8 kDa, on SDS-PAGE under reducing conditions.
      
			      		
		
      
		
      
			
			
      AA Sequence
      
			      		
			
			
      V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA
      
			      		
		
      
		
      
			
			
      Tag
      
			      		
			
			
      None
      
			      		
		
      
		
      
			
			
      Physical Appearance
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 95% as determined by SDS-PAGE
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized after extensive dialysis against PBS.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH?O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt.
      
1week, 2 to 8 °C under sterile conditions after reconstitution.
      
3 months, -20 °C under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      Cautions
      


      1.For your safety and health, please wear lab coats and disposable gloves for operation.
      


      2.For research use only!
      


      HB230921
      

         
       
       
       
       
       
       
       
      400-6111-883