Recombinant Human BAFFR/TNFRSF13C(His Tag)
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產(chǎn)品說(shuō)明書
FAQ
COA
已發(fā)表文獻(xiàn)
B-cell activating factor (BAFF) is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation (1-4). BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL (a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.
產(chǎn)品性質(zhì)
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別名（Synonyms） |
CD268; BAFFR; BR3; TNFRSF13C; BROMIX; CVID4; prolixin |
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Uniprot No. |
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Recombinant Human BAFFR/TNFRSF13C Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ser7-Ala71. |
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分子量(Molecular Weight) |
The protein has a predicted MW of 7.6 kDa. Due to glycosylation, the protein migrates to 13-25 kDa based on Tris-Bis PAGE result. |
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外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE |
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活性（Activity） |
1.ELISA Data: Immobilized Human BAFFR, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. 2.ELISA Data: Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 2.3μg/ml determined by ELISA. 3.SPR Data: Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF Trimer, hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay (Biacore T200). |
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內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| 復(fù)溶（Reconstitution） | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220624
