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      Recombinant Human LIGHT/TNFSF14(His Tag)
       
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      產(chǎn)品信息
      


      
	
		
      
			
			
      產(chǎn)品名稱
      
			      		
			
			
      產(chǎn)品編號
      
			      		
			
			
      規(guī)格
      
			      		
		
      
		
      
			
			
      Recombinant Human LIGHT / TNFSF14 
      
			      		
			
			
      93747ES25
      
			      		
			
			
      25 μg
      
			      		
		
      
		
      
			
			
      93747ES60
      
			      		
			
			
      100 μg
      
			      		
		
      
		
      
			
			
      93747ES76
      
			      		
			
			
      500 μg
      
			      		
		
      
	      

      


       
      


      產(chǎn)品描述
      


      LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.
      


       
      


      產(chǎn)品性質(zhì)
      


      
	
		
      
			
			
      別名（Synonyms）
      
			      		
			
			
      CD258, TNFSF14, HVEM-L, LIGHT, LTg, TR2
      
			      		
		
      
		
      
			
			
      Uniprot No.
      
			      		
			
			
      O43557-1
      
			      		
		
      
		
      
			
			
      表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
      
			      		
			
			
      HEK293 Cells-derived Human LIGHT / TNFSF14 Protein, Asp74-Val240 with hFc tag at the N-terminal
      
			      		
		
      
		
      
			
			
      分子量(Molecular Weight)
      
			      		
			
			
      Approximately 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
      
			      		
		
      
		
      
			
			
      外觀(Physical Appearance）
      
			      		
			
			
      Sterile Filtered White lyophilized (freeze-dried) powder.
      
			      		
		
      
		
      
			
			
      純度（Purity）
      
			      		
			
			
      > 95% as determined by SDS-PAGE and HPLC
      
			      		
		
      
		
      
			
			
      活性（Activity）
      
			      		
			
			
      1.ELISA Data: Immobilized Human LIGHT, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12 μg/mL determined by ELISA.
      

			
      2.ELISA Data: Immobilized Human LIGHT, His Tag at 0.5 μg/ml (100 μL/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 of 22.9 ng/mL determined by ELISA.
      
			      		
		
      
		
      
			
			
      內(nèi)毒素（Endotoxin）
      
			      		
			
			
      < 1.0 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      制劑（Formulation）
      
			      		
			
			
      Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
      
			      		
		
      
		
      
			
			
      復(fù)溶（Reconstitution）
      
			      		
			
			
      Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
      
			      		
		
      
	      

      


       
      


      運輸與保存方法
      


      冰袋運輸。-20℃至-80℃保存，一年有效期。
      


      復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。
      

      


      

      建議第一次使用時分裝凍存，避免反復(fù)凍融。
      


      

       
      


      注意事項
      


        
	
      1. 避免反復(fù)凍融。
        2. 
	
      2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
        3. 
	
      3. 本產(chǎn)品僅作科研用途！
        4. 

      


       
      


      產(chǎn)品數(shù)據(jù)
      


      Tris-Bis PAGE
      


      


       
      


      SEC-HPLC
      


      

      


      ELISA Data
      


       
      


       
      


      ELISA Data
      


       
      

      

         
       
       
       
       
       
       
       
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