產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC. M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis. In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear.
產(chǎn)品性質(zhì)
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別名（Synonyms） |
CSF1R, CSF-1-R, CSFR, FMSFIM2, MCSFR, M-CSFR, C-FMS, FIM2, FMS, HDLS |
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Uniprot No. |
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
HEK293 Cells-derived Human M-CSF R / CSF1R / CD115 Protein, Ile20-Glu512 with hFc tag at the C-terminal |
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分子量(Molecular Weight) |
Approximately 81.3 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Tris-Bis PAGE result. |
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外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
1.ELISA Data: Immobilized Human M-CSF at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 of 12.4 ng/mL determined by ELISA. 2.SPR Data: Human M-CSF, His Tag captured on CM5 Chip via Anti-his Antibody can bind Human M-CSF R, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). |
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內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復(fù)溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220629
