Recombinant Human Fc gamma RIIIB/CD16b (NA1)(His Tag)
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產(chǎn)品說明書
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Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes.
產(chǎn)品性質(zhì)
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別名（Synonyms） |
CD16, FCG3, FCGR3B, FCRIIIB, IGFR3, CD16b (NA2), FCG3B, FCG3, FCGR3, FCR-10 |
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Uniprot No. |
AAA35881.1 |
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Recombinant Human Fc gamma RIIIB / CD16b (NA1) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gly17-Ser200. |
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分子量(Molecular Weight) |
Approximately 22 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result. |
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外觀(Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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純度（Purity） |
> 95% as determined by SDS-PAGE and HPLC |
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活性（Activity） |
SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 μM as determined in SPR assay (Biacore T200). |
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內(nèi)毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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復(fù)溶（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1.避免反復(fù)凍融。
2.為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
3.本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220707
