Recombinant Human Fas/TNFRSF6/CD95 (hFc Tag)
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產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
Fas, also known as APO?1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. All appear to block the extrinsic apoptosis pathway induced by the Fas ligand (FasL, TNFSF6), a type II transmembrane protein of the TNF family that can be expressed on activated T?lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form. Engagement of FAS induces oligomerization of preformed Fas trimers. The activated receptor recruits the adaptor molecule FADD to form the Death?Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade. Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation?induced death of T lymphocytes that terminates the immune reaction. In immune?privileged tissues, infiltrating Fas?bearing lymphocytes and inflammatory cells are killed by FasL engagement. Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity. The Fas pathway also appears to cross?communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway.
產(chǎn)品性質(zhì)
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別名 |
FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR |
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Uniprot No. |
P25445.1 |
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表達(dá)區(qū)間及表達(dá)系統(tǒng) |
Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Gln26-Asn173. |
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分子量 |
Approximately 43.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
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純度 |
> 95% as determined by SDS-PAGE and HPLC. |
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活性 |
ELISA Data：Immobilized Human Fas Ligand, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. |
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內(nèi)毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制劑 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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復(fù)溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。
復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。
建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。
注意事項(xiàng)
1. 避免反復(fù)凍融。
2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3. 本產(chǎn)品僅作科研用途！
產(chǎn)品數(shù)據(jù)
HB220810
