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      Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
       
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      產(chǎn)品說(shuō)明書
                  
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      This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.
      


      This product is produced in accordance with GMP process requirements, and the product is provided in liquid form
      


       
      


      Product Properties
      


      
	
		
      
			
			
      Source
      
			      		
			
			
      Recombinant E.coli with Pyrophosphatase gene from yeast
      
			      		
		
      
		
      
			
			
      Optimum Temperature
      
			      		
			
			
      25℃
      
			      		
		
      
		
      
			
			
      Storage Buffer
      
			      		
			
			
      50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
      
			      		
		
      
		
      
			
			
      Unit Definition
      
			      		
			
			
      One unit (U) is the amount of enzyme needed to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）
      
			      		
		
      
	      

      


       
      


      Contents
      


      
	
		
      
			
			
      Catalog No.
      
			      		
			
			
      Name
      
			      		
			
			
      Catalog No./Specification
      
			      		
		
      
		
      
			
			
      10672ES03
      

			
      (1 U)
      
			      		
			
			
      10672ES10
      

			
      (10 U)
      
			      		
			
			
      10672ES60
      

			
      (100 U)
      
			      		
		
      
		
      
			
			
      10672
      
			      		
			
			
      Pyrophosphatase, Inorganic GMP-grade (0.1 U/μL)
      
			      		
			
			
      10 μL
      
			      		
			
			
      100 μL
      
			      		
			
			
      1 mL
      
			      		
		
      
	      

      


       
      


      Shipping and Storage
      


      The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
      


       
      


      Notes
      


      1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
      


      2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.
      


      3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this
      


       
      


      HB220701
      

         
       
       
       
       
       
       
       
      400-6111-883