Peroxidase AffiniPure Goat Anti-Mouse IgG(H+L) 過(guò)氧化物酶標(biāo)記山羊抗小鼠IgG(H+L)
批量采購(gòu)， 請(qǐng)點(diǎn)擊詢價(jià)
產(chǎn)品說(shuō)明書(shū)
FAQ
COA
已發(fā)表文獻(xiàn)
產(chǎn)品描述
本品是由辣根過(guò)氧化酶（HRP）標(biāo)記的山羊抗小鼠IgG(H+L)，使用抗原偶聯(lián)的瓊脂糖微珠從山羊抗血清內(nèi)親和色譜純化所得。免疫電泳和/或ELISA法檢測(cè)顯示本品特異性結(jié)合完整的小鼠IgG分子，也會(huì)與其他小鼠免疫球蛋白的輕鏈結(jié)合，可能會(huì)與其他物種免疫球蛋白發(fā)生交叉反應(yīng)，但不會(huì)識(shí)別非免疫球蛋白類的血清蛋白。
本品適合做單標(biāo)實(shí)驗(yàn)。要做多標(biāo)（Multiple-labeling）實(shí)驗(yàn)，建議使用與相近物種血清蛋白或者免疫球蛋白預(yù)先經(jīng)過(guò)親和吸附處理的二抗。
翌圣為您提供Western Blot實(shí)驗(yàn)整體解決方案，相關(guān)產(chǎn)品選購(gòu)請(qǐng)參考：WB實(shí)驗(yàn)系列產(chǎn)品-選購(gòu)指南
產(chǎn)品應(yīng)用
建議稀釋濃度：
IHC/ICC：1:250–1:2500；
ELISA/WB（顯色法）：1:2500–1:50000
ELISA/WB（化學(xué)發(fā)光法）：1:5000–1:100000
產(chǎn)品性質(zhì)
|
抗體濃度（Antibody Concentration） |
100 µL (0.4 mg/mL) |
|
緩沖液（Buffer） |
0.005 M磷酸鈉，0.125 M氯化鈉，pH 7.6 |
|
穩(wěn)定劑（Stabilizer） |
7.5 mg/mL BSA（無(wú)IgG，蛋白酶），50%甘油 |
|
防腐劑（Preservative） |
None（疊氮化鈉會(huì)抑制HRP活性） |
|
原料來(lái)源（Source of Material） |
Jackson Immunoresearch 115-035-003 |
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃分裝保存，盡量避免反復(fù)凍融。有效期1年。
注意事項(xiàng)
1）本品不能保存于疊氮化鈉中，其會(huì)抑制辣根過(guò)氧化酶（HRP）活性；
2）為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
3）本產(chǎn)品僅作科研用途！
HB210715
暫無(wú)內(nèi)容
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