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      Recombinant Mouse Fc gamma RIIB/CD32b Protein,His Tag
       
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      產(chǎn)品說(shuō)明書(shū)
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
      性能參數(shù)
      


      
	
		
      
			
			
      表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
      
			      		
			
			
      Recombinant Mouse Fc gamma RIIB/CD32b Protein is expressed from HEK293 with His tag at the C-terminal. It contains Thr40-Arg217.[Uniprot | NP_001070657.1]
      
			      		
		
      
		
      
			
			
      分子量大小（Molecular Weight）
      
			      		
			
			
      The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
      
			      		
		
      
		
      
			
			
      內(nèi)毒素（Endotoxin）
      
			      		
			
			
      Less than 1EU per ug by the LAL method.
      
			      		
		
      
		
      
			
			
      純度（Purity）
      
			      		
			
			
      > 95% as determined by Tris-Bis PAGEand  HPLC
      
			      		
		
      
		
      
			
			
      活性（Activity）
      
			      		
			
			
      SPR Data: OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 μM as determined in SPR assay
      
			      		
		
      
		
      
			
			
      制劑（Formulation）
      
			      		
			
			
      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
      
			      		
		
      
		
      
			
			
      重構(gòu)方法（Reconstitution）
      
			      		
			
			
      Centrifuge tubes before opening. Reconstituting to a concentration more than 100
      

			
       μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
      
			      		
		
      
	      

      


       
      


      儲(chǔ)存條件
      


      The product should be stored at -25~-15℃ for 1 year from date of receipt.
      


      2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項(xiàng)
      


      1.Please operate with lab coats and disposable gloves,for your safety.
      


      2.This product is for research use only.   
      


       
      


      Ver. CN20230522
      

         
       
       
       
       
       
       
       
      400-6111-883