表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human IL-2 R beta/CD122 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala27-Asp239.[Accession | NP_000869]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

SPR Data 1: Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 0.079 μM as determined in SPR assay.

SPR Data 2: Biotinylated Human IL-2 R beta, His-Avi Tag captured on CM5 Chip via Anti-His

Antibody can bind Human IL-15, No Tag with an affinity constant of 12.91 nM as determined

in SPR assay.

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.