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      Recombinant Human IL-13Ra1 Protein,hFc Tag
       
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      產(chǎn)品說明書
                  
      FAQ
                  
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      表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
      
			      		
			
			
      Human IL-13Ra1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ala27-Thr343.[Accession | P78552]
      
			      		
		
      
		
      
			
			
      分子量大?。?/font>Molecular Weight）
      
			      		
			
			
      The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result.
      
			      		
		
      
		
      
			
			
      內(nèi)毒素（Endotoxin）
      
			      		
			
			
      Less than 1EU per μg by the LAL method.
      
			      		
		
      
		
      
			
			
      純度（Purity）
      
			      		
			
			
      > 95% as determined by Tris-Bis PAGE and HPLC
      
			      		
		
      
		
      
			
			
      活性（Activity）
      
			      		
			
			
      ELISA Data:Immobilized Human IL-13, His Tag at 5μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7μg/ml determined by ELISA   
      

			
      SPR Data:Human IL-13Ra1, hFc Tag captured on Protein A chip, can bind Human IL-13, His Tag with an affinity constant of 53.1nM as determined in a SPR assay.
      
			      		
		
      
		
      
			
			
      制劑（Formulation）
      
			      		
			
			
      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
      
			      		
		
      
		
      
			
			
      重構(gòu)方法（Reconstitution）
      
			      		
			
			
      Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
      
			      		
		
      
	      

      


       
      


      儲存條件
      


      The product should be stored at -25~-15℃ for 1 year from date of receipt.
      


      2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項(xiàng)
      


      1.Please operate with lab coats and disposable gloves,for your safety.
      


      2.This product is for research use only.
      


      Ver. CN20230625
      

         
       
       
       
       
       
       
       
      400-6111-883