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      Recombinant Human/Murine/Rat Activin A Protein
       
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      產(chǎn)品說明書
                  
      FAQ
                  
      COA
                  
      已發(fā)表文獻(xiàn)
                  
       
       
      


      Activin A, a homodimer of activin βA subunits, is a member of the transforming growth factor (TGF)-β super-family. Activin A is produced by the placenta during human pregnancy, has been recognized as a multifunctional cytokine expressed in a wide range of tissues and cells with roles in regulation of wound repair, cell differentiation, apoptosis, embryogenesis, and inflammation. 
      


       
      


      性能參數(shù)
      


      
	
		
      
			
			
      Synonyms
      
			      		
			
			
      Activin A, activin AB alpha polypeptide, inhibin beta A chain
      
			      		
		
      
		
      
			
			
      Uniprot No.
      
			      		
			
			
      P08476
      
			      		
		
      
		
      
			
			
      Source
      
			      		
			
			
      Human/Murine/Rat Activin A Protein is expressed from CHO cell.It contains Gly311 - Ser426.
      
			      		
		
      
		
      
			
			
      Molecular Weight
      
			      		
			
			
      Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains.
      
			      		
		
      
		
      
			
			
      Biological Activity
      
			      		
			
			
      Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Activin A is approximately 1 IU/μg.
      
			      		
		
      
		
      
			
			
      Purity
      
			      		
			
			
      > 95% as determined by SDS-PAGE.
      
			      		
		
      
		
      
			
			
      Endotoxin
      
			      		
			
			
      <0.01 EU per 1μg of the protein by the LAL method.
      
			      		
		
      
		
      
			
			
      Formulation
      
			      		
			
			
      Lyophilized from 0.2 μm filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA.
      
			      		
		
      
		
      
			
			
      Reconstitution
      
			      		
			
			
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions.
      
			      		
		
      
	      

      


       
      


      儲存條件
      


      The product should be stored at -25~-15℃ for 1 year from date of receipt.
      


      1 month, 2 ~8 °C under sterile conditions after reconstitution.
      


      3 months, -25~-15℃ under sterile conditions after reconstitution.
      


      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
      


       
      


      注意事項(xiàng)
      


      1.Please operate with lab coats and disposable gloves,for your safety.
      


      2.This product is for research use only. 
      


      Ver. CN20230424
      

         
       
       
       
       
       
       
       
      400-6111-883