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      Recombinant Human VEGF165 Protein (HEK293)

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

       

      VEGF 165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF. 

       

      性能參數(shù)

      Synonyms

      VEGF-165;VEGF; VEGFA; MVCD1;  VPF; RP1-261G23.1;

      Uniprot No.

      P15692-4

      Source

      Recombinant Human VEGF165 Protein is expressed from HEK293 without tag. It contains Ala27-Arg191

      Molecular Weight

      The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.

      Biological Activity

      ED50 < 3 ng/ml, measured in a cell proliferation assay using HUVEC cells.

      Purity

      > 95% by SDS-PAGE and HPLC

      Endotoxin

      <1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

      Reconstitution

      It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

       

      儲(chǔ)存條件

      The product should be stored at -25~-15℃ for 1 year from date of receipt.

      2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

      3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      注意事項(xiàng)

      1.Please operate with lab coats and disposable gloves,for your safety.

      2.This product is for research use only.

      Ver. CN20231218

      400-6111-883