產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
性能參數(shù)
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分子別名（Synonyms） |
Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451 |
|
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Cynomolgus IL-10 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Ser19-Asn178.[Accession | G7NVB0] |
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分子量大小（Molecular Weight） |
The protein has a predicted MW of 19.78 kDa. Due to glycosylation, the protein migrates to 20-22 kDa based on SDS-PAGE result. |
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內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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純度（Purity） |
> 95% as determined by SDS-PAGE |
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活性（Activity） |
ELISA Data: Immobilized Cynomolgus IL-10, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-10R alpha, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA . |
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制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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重構(gòu)方法（Reconstitution） |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
1.The product should be stored at -25~-15℃ for 1 year from date of receipt.
2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
