批量采購, 請點(diǎn)擊詢價(jià)
性能參數(shù)
分子別名(Synonyms) |
ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2 |
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source) |
Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr70-Asp316.[Accession | AAC40113.1] |
分子量大?。∕olecular Weight) |
The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on SDS-PAGE result. |
內(nèi)毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
純度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
活性(Activity) |
ELISA Data: Immobilized Mouse RANKL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 1.8ng/ml determined by ELISA. |
制劑(Formulation) |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外觀(Appearance) |
凍干粉 |
儲(chǔ)存條件
-25~-15℃保存,收到貨之后有效期1年。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。
復(fù)溶方法
開蓋之前,先離心。使用蒸餾水復(fù)溶凍干的蛋白質(zhì),復(fù)溶濃度超過100 μg/mL。建議首次使用時(shí)將蛋白分裝為每次實(shí)驗(yàn)用量,避免反復(fù)凍融。
注意事項(xiàng)
1.為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。
2.本產(chǎn)品僅作科研用途。