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Fetal Bovine Serum Gold 胎牛血清(特級)

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產(chǎn)品描述

Yeasen胎牛血清（特級），經(jīng)3次100 nm過濾和支原體、病毒篩查，經(jīng)過嚴(yán)格檢驗(yàn)測試后全自動化罐裝，規(guī)范化生產(chǎn)，品質(zhì)保證，含有豐富的細(xì)胞生長所需的營養(yǎng)成分，適用于培養(yǎng)大部分常規(guī)細(xì)胞系。

產(chǎn)品性質(zhì)

中文別名（Chinese Synonym）	胎牛血清（特級）
英文別名（English Synonym）	Fetal Bovine Serum Gold
內(nèi)毒素水平	≤5 EU/mL
血紅蛋白含量	≤0.02%(w/v)
支原體檢測	陰性
滅菌處理	三次100 nm過濾

產(chǎn)品選購指南

1. 適用于絕大多數(shù)常規(guī)細(xì)胞的培養(yǎng)，如常規(guī)腫瘤細(xì)胞、規(guī)?；a(chǎn)細(xì)胞（293T、CHO、VERO等）、免疫細(xì)胞（RAW264.7、THP-1等）。其他嬌貴細(xì)胞或難培養(yǎng)的細(xì)胞（如干細(xì)胞（如NSC、PB-MSCs等）、難培養(yǎng)的原代細(xì)胞（上皮細(xì)胞、內(nèi)皮細(xì)胞、心肌細(xì)胞等）、其他難培養(yǎng)的細(xì)胞（如部分乳腺癌細(xì)胞、部分結(jié)直腸癌細(xì)胞、部分肺腺癌細(xì)胞、部分膠質(zhì)瘤細(xì)胞等）建議選用40131ES、40132ES。

2. 多種生長因子，細(xì)胞培養(yǎng)狀態(tài)穩(wěn)定。

3. 純天然制品，不含任何人為添加成分。

4. 為避免反復(fù)凍融，可以選購小規(guī)格（50 mL）產(chǎn)品，即用即取，方便快捷。10個50 mL產(chǎn)品可等同于500 mL產(chǎn)品。

產(chǎn)品優(yōu)勢

1. 兼容范圍廣——適用于多種常規(guī)細(xì)胞培養(yǎng)

2. 產(chǎn)品純天然——對標(biāo)進(jìn)口胎牛血清，營養(yǎng)豐富，不含任何人為添加成分

3. 細(xì)胞狀態(tài)好——多種生長因子，批次穩(wěn)定，細(xì)胞培養(yǎng)狀態(tài)穩(wěn)定

4. 小規(guī)格便利——50 mL便攜裝，即用即取，避免反復(fù)凍融，避免沉淀產(chǎn)生，避免分裝污染，方便快捷，產(chǎn)品升級不加價(jià)

5. 產(chǎn)品質(zhì)控嚴(yán)——經(jīng)過三次0.1 μM無菌過濾，有效去除微生物，通過支原體和病毒篩查檢測

6. 內(nèi)毒素極低——內(nèi)毒素≤5 EU/mL，內(nèi)毒素含量低，完全符合國家質(zhì)量標(biāo)準(zhǔn)

7. 客戶群體廣——使用課題組數(shù)多達(dá)4000+，如中科院上海生命科學(xué)研究院，清華大學(xué)，浙江大學(xué)，上海交通大學(xué)等各大高校和研究院

8. 發(fā)表文獻(xiàn)多——客戶發(fā)表的文章引用影響因子累計(jì)高達(dá)1000+

產(chǎn)品應(yīng)用案例（細(xì)胞培養(yǎng)）

產(chǎn)品應(yīng)用案例（單克隆實(shí)驗(yàn)）

運(yùn)輸和保存方法

運(yùn)輸方式：干冰運(yùn)輸；

保存方式：-20℃至-10℃可保存5年。

【注】一旦解凍，血清應(yīng)該保存在2℃到8℃冰箱，存儲時間不宜超過6周。如果需要長期保存，建議將血清在無菌環(huán)境下先進(jìn)行分裝，后重新冷凍保存，避免反復(fù)凍融。

解凍的方式（二選一）

1) 將血清從-20℃存儲條件下取出，放置于2-8℃冰箱中過夜，使其部分溶解，然后在室溫條件下使其全部融解，溶解過程中須不時的搖動瓶身混合里面的液體。建議采取此種方式解凍更優(yōu)。

2) 直接將血清從-20℃取出后，放置于37℃水浴中，不斷搖晃瓶身使其加快解凍和混合。解凍以后不要在37℃水浴放置太長時間。

【注】如果不晃動瓶身，當(dāng)溫度超過40℃的時候沉積在瓶底的物質(zhì)有可能會發(fā)生蛋白變形，出現(xiàn)沉淀。如果出現(xiàn)沉淀，建議可將血清分裝到無菌離心管中，400~600 g（如500 g）離心5 min，取血清上清液，加入到基礎(chǔ)培養(yǎng)基中，再一起過濾，全程保證無菌環(huán)境即可。

注意事項(xiàng)

1）使用過程中，避免反復(fù)凍融，避免紫外線照射。

2）避免血清在37℃環(huán)境中時間過長。

3）避免血清在2~8℃條件下長期保存，可以將血清無菌分裝后，置于-20℃的環(huán)境中長期保存。

4）避免血清在室溫條件下過長時間放置。

5）為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。

6）本產(chǎn)品僅作科研用途！

客戶使用本產(chǎn)品發(fā)表的科研文獻(xiàn)（部分）

[1] Hu S, Peng L, Xu C, Wang Z, Song A, Chen FX. SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. Mol Cell. 2021;81(21):4425-4439.e6. doi:10.1016/j.molcel.2021.08.029 (IF:17.970)

[2] Teng KX, Niu LY, Xie N, Yang QZ. Supramolecular photodynamic agents for simultaneous oxidation of NADH and generation of superoxide radical. Nat Commun. 2022;13(1):6179. Published 2022 Oct 19. doi:10.1038/s41467-022-33924-3 (IF:17.694)

[3] Tu J, Li W, Yang S, et al. Single-Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression. Adv Sci (Weinh). 2022;9(3):e2103631. doi:10.1002/advs.202103631 (IF:16.806)

[4] Zhang W, Liu J, Li X, et al. Precise Chemodynamic Therapy of Cancer by Trifunctional Bacterium-Based Nanozymes. ACS Nano. 2021;15(12):19321-19333. doi:10.1021/acsnano.1c05605 (IF:15.881)

[5] Zhao C, Xie Y, Xu L, et al. Structures of a mammalian TRPM8 in closed state. Nat Commun. 2022;13(1):3113. Published 2022 Jun 3. doi:10.1038/s41467-022-30919-y (IF:14.919)

[6] Cai Z, Zhang Y, Zhang W, et al. Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biol. 2022;52:102321. doi:10.1016/j.redox.2022.102321 (IF:11.799)

[7] Zhai Y, Liu M, Yang T, et al. Self-activated arsenic manganite nanohybrids for visible and synergistic thermo/immuno-arsenotherapy. J Control Release. 2022;350:761-776. doi:10.1016/j.jconrel.2022.08.054 (IF:11.467)

[8] Liu M, Chen C, Yu J, et al. The gelatin-based liquid marbles for cell cryopreservation. Mater Today Bio. 2022;17:100477. Published 2022 Oct 31. doi:10.1016/j.mtbio.2022.100477 (IF:10.761)

[9] Li S, Zhang J, Qian S, et al. S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer. Cancer Commun (Lond). 2021;41(2):154-170. doi:10.1002/cac2.12130 (IF:10.392)

產(chǎn)品應(yīng)用

胎牛血清（特級）40130ES適用細(xì)胞系（不完全統(tǒng)計(jì)）
序號	物種	細(xì)胞名稱	中文名稱
1	人	CCRF-CEM	人急性淋巴細(xì)胞白血病T淋巴細(xì)胞
2	人	A-431	人皮膚鱗癌細(xì)胞
3	小鼠	SP2/0	小鼠骨髓瘤細(xì)胞
4	人	CFPAC-1	人胰腺癌細(xì)胞
5	人	6T-CEM	人T細(xì)胞白血病細(xì)胞
6	人	T84	人結(jié)直腸癌細(xì)胞
7	人	Hela 229	人宮頸癌細(xì)胞
8	人	Huh-7	人肝癌細(xì)胞
9	人	PC-3	人前列腺癌細(xì)胞
10	人	2BS	人肺二倍體細(xì)胞
11	人	Jurkat E6-1	人急性T淋巴細(xì)胞白血病細(xì)胞
12	人	HCCLM3	高轉(zhuǎn)移人肝癌細(xì)胞
13	人	MRC-5	人肺二倍體細(xì)胞
14	人	MOLM-13	人急性髓系白血病細(xì)胞
15	人	NCI-H209	人小細(xì)胞肺癌細(xì)胞
16	人	MSC	人臍帶間充質(zhì)干細(xì)胞
17	人	NCI-H1650	人非小細(xì)胞肺癌細(xì)胞
18	人	NCI-H1395	人肺腺癌細(xì)胞
19	大鼠	RH-35	大鼠肝癌細(xì)胞
20	人	BT-474	人乳腺導(dǎo)管癌細(xì)胞
21	人	PANC-1	人胰腺癌細(xì)胞
22	人	SH-SY5Y	人神經(jīng)母細(xì)胞瘤
23	人	HCC 94	人子宮鱗癌細(xì)胞
24	人	ME-180	人子宮頸表皮癌細(xì)胞
25	人	786-O	人腎細(xì)胞腺癌細(xì)胞
26	人	MG-63	人骨肉瘤細(xì)胞
27	人	RBE	人肝膽管癌細(xì)胞
28	小鼠	MKWFCs	小鼠表皮角質(zhì)形成細(xì)胞
29	人	BT-549	人乳腺管癌細(xì)胞
30	人	COLO 320DM	人結(jié)直腸腺癌細(xì)胞
31	人	ES-2	人卵巢透明細(xì)胞癌
32	人	HAL-01	人淋巴細(xì)胞白血病細(xì)胞
33	人	HCT-8	人回盲腸癌細(xì)胞
34	人	Hep3B2-1-7	人肝癌細(xì)胞
35	人	Hs-683	人腦神經(jīng)膠質(zhì)瘤細(xì)胞
36	人	K-562	人慢性髓原白血病細(xì)胞
37	人	WI-38	人胚肺成纖維細(xì)胞
38	大鼠	NRK	大鼠腎細(xì)胞
39	大鼠	PC-12(低分化)	大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞(低分化)
40	大鼠	PC-12(高分化)	大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞(高分化)
41	人	KG-1	人急性骨髓性白血病細(xì)胞
42	人	ARPE-19	人視網(wǎng)膜上皮細(xì)胞
43	人	Farage	人B淋巴瘤細(xì)胞
44	人	NK92	人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞
45	人	SW-13	人腎上腺皮質(zhì)小細(xì)胞癌細(xì)胞
46	人	A3	人T淋巴細(xì)胞白血病細(xì)胞
47	人	ZR-75-30	人乳腺癌細(xì)胞
48	人	SNU-387	人肝癌細(xì)胞
49	人	SK-MEL-5	人皮膚黑色素瘤細(xì)胞
50	人	MCF7	人乳腺癌細(xì)胞
51	人	MDA-MB-436	人乳腺腺癌細(xì)胞
52	人	MKN74	人胃癌細(xì)胞
53	人	NCI-H1437	人肺癌細(xì)胞
54	人	NCI-H524	人非小細(xì)胞肺癌細(xì)胞
55	人	NCI-H23	人肺癌細(xì)胞
56	人	NCI-H661	人大細(xì)胞肺癌細(xì)
57	人	LM3	肝癌細(xì)胞株
58	人	NCI-H508	人結(jié)腸癌細(xì)胞
59	人	PA-1	人卵巢畸胎瘤細(xì)胞
60	人	RG2 [D74]	大鼠膠質(zhì)瘤細(xì)胞
61	小鼠	bEnd.3	小鼠腦微血管內(nèi)皮細(xì)胞
62	人	MHCC-97H	人高轉(zhuǎn)移潛能肝癌細(xì)胞
63	小鼠	MB49	小鼠膀胱癌細(xì)胞
64	小鼠	ID8	小鼠卵巢上皮癌細(xì)胞
65	小鼠	HC11	小鼠乳腺上皮細(xì)胞
66	小鼠	MC38	小鼠結(jié)腸癌細(xì)胞
67	小鼠	AML12	小鼠肝細(xì)胞
68	人	hccc-9810	人膽管細(xì)胞型肝癌細(xì)胞
69	人	Hep G2	人肝癌細(xì)胞
70	人	Calu-6	人退行性癌細(xì)胞
71	人	MM.1S	人多發(fā)性骨髓瘤細(xì)胞
72	小鼠	P19	小鼠畸胎瘤細(xì)胞
73	人	PC-9	人肺癌細(xì)胞
74	人	NCI-H226	人肺鱗癌細(xì)胞
75	人	NCI-H929	人骨髓瘤細(xì)胞

客戶使用本產(chǎn)品的數(shù)據(jù)展示

HB250114

[1] Hu S, Peng L, Xu C, Wang Z, Song A, Chen FX. SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape. Mol Cell. 2021;81(21):4425-4439.e6. doi:10.1016/j.molcel.2021.08.029(IF:17.970)
[2] Tu J, Li W, Yang S, et al. Single-Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression. Adv Sci (Weinh). 2022;9(3):e2103631. doi:10.1002/advs.202103631(IF:16.806)
[3] Zhang W, Liu J, Li X, et al. Precise Chemodynamic Therapy of Cancer by Trifunctional Bacterium-Based Nanozymes. ACS Nano. 2021;15(12):19321-19333. doi:10.1021/acsnano.1c05605(IF:15.881)
[4] Zhao C, Xie Y, Xu L, et al. Structures of a mammalian TRPM8 in closed state. Nat Commun. 2022;13(1):3113. Published 2022 Jun 3. doi:10.1038/s41467-022-30919-y(IF:14.919)
[5] Cai Z, Zhang Y, Zhang W, et al. Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biol. 2022;52:102321. doi:10.1016/j.redox.2022.102321(IF:11.799)
[6] Li S, Zhang J, Qian S, et al. S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer. Cancer Commun (Lond). 2021;41(2):154-170. doi:10.1002/cac2.12130(IF:10.392)
[7] Yang X, Qiu Q, Liu G, et al. Traceless antibiotic-crosslinked micelles for rapid clearance of intracellular bacteria. J Control Release. 2022;341:329-340. doi:10.1016/j.jconrel.2021.11.037(IF:9.776)
[8] Wang X, Qi Y, Wang Z, et al. RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex. Cell Rep. 2022;39(4):110732. doi:10.1016/j.celrep.2022.110732(IF:9.423)
[9] Ma Z, Zhang Y, Zhang J, et al. Ultrasmall Peptide-Coated Platinum Nanoparticles for Precise NIR-II Photothermal Therapy by Mitochondrial Targeting. ACS Appl Mater Interfaces. 2020;12(35):39434-39443. doi:10.1021/acsami.0c11469(IF:8.758)
[10] Sun Q, Ye Y, Gui A, et al. MORTALIN-Ca²⁺ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. Cancer Lett. 2022;537:215678. doi:10.1016/j.canlet.2022.215678(IF:8.679)
[11] Wang Y, Sun Q, Ye Y, et al. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. JCI Insight. 2022;7(10):e157874. Published 2022 May 23. doi:10.1172/jci.insight.157874(IF:8.315)
[12] Wang Y, Sun Q, Ye Y, et al. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. JCI Insight. 2022;7(10):e157874. Published 2022 May 23. doi:10.1172/jci.insight.157874(IF:8.315)
[13] Wang Y, Sun Q, Ye Y, et al. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis. JCI Insight. 2022;7(10):e157874. Published 2022 May 23. doi:10.1172/jci.insight.157874(IF:8.315)
[14] Liu Z, Tao C, Li S, et al. circFL-seq reveals full-length circular RNAs with rolling circular reverse transcription and nanopore sequencing. Elife. 2021;10:e69457. Published 2021 Oct 14. doi:10.7554/eLife.69457(IF:8.146)
[15] Cao J, Peng X, Li H, et al. Ultrasound-assisted continuous-flow synthesis of PEGylated MIL-101(Cr) nanoparticles for hematopoietic radioprotection. Mater Sci Eng C Mater Biol Appl. 2021;129:112369. doi:10.1016/j.msec.2021.112369(IF:7.328)
[16] Ma H, Lin J, Li L, et al. Formaldehyde reinforces pro-inflammatory responses of macrophages through induction of glycolysis. Chemosphere. 2021;282:131149. doi:10.1016/j.chemosphere.2021.131149(IF:7.086)
[17] Chen Y, Chen Y, Jiang X, et al. Vascular Adventitial Fibroblasts-Derived FGF10 Promotes Vascular Smooth Muscle Cells Proliferation and Migration in vitro and the Neointima Formation in vivo. J Inflamm Res. 2021;14:2207-2223. Published 2021 May 25. doi:10.2147/JIR.S305204(IF:6.922)
[18] Wang Y, Zhao M, Li W, et al. BMSC-Derived Small Extracellular Vesicles Induce Cartilage Reconstruction of Temporomandibular Joint Osteoarthritis via Autotaxin-YAP Signaling Axis. Front Cell Dev Biol. 2021;9:656153. Published 2021 Apr 1. doi:10.3389/fcell.2021.656153(IF:6.684)
[19] Liu W, Wu Z, Yu Y, et al. Functional Evaluation of KEL as an Oncogenic Gene in the Progression of Acute Erythroleukemia. Oxid Med Cell Longev. 2022;2022:5885342. Published 2022 Jan 30. doi:10.1155/2022/5885342(IF:6.543)
[20] Wang G, Wang H, Jin Y, et al. Galactooligosaccharides as a protective agent for intestinal barrier and its regulatory functions for intestinal microbiota. Food Res Int. 2022;155:111003. doi:10.1016/j.foodres.2022.111003(IF:6.475)
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