產(chǎn)品說明書
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產(chǎn)品簡介
DMEM/F-12（Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 Ham）是DMEM培養(yǎng)基和Ham's F-12培養(yǎng)基1:1的混合物，支持多種哺乳動物細胞的生長，包括神經(jīng)膠質(zhì)細胞、成纖維細胞、MDCK、人內(nèi)皮細胞和大鼠的成纖維細胞等細胞的生長。
DMEM是改良的Eagle基本培養(yǎng)基（BME），維生素和氨基酸濃度是BME的四倍，Ham's F-12是以Ham's F-10培養(yǎng)基為基礎(chǔ)，提高了膽堿、肌醇、腐胺和幾種氨基酸的濃度。
L-谷氨酰胺是細胞培養(yǎng)液體環(huán)境中所必需的一種營養(yǎng)成分，丙酮酸鈉可以作為細胞培養(yǎng)中的替代碳源，在葡萄糖不足的情況下，細胞也可以代謝丙酮酸鈉。
本產(chǎn)品含有：L-谷氨酰胺、丙酮酸鈉、酚紅、HEPES。
產(chǎn)品信息
|
貨號 |
41406ES76 |
|
規(guī)格 |
500 mL |
|
外觀 |
紅色澄清透明液體 |
|
無菌 |
無菌過濾 |
|
pH |
7.0-7.4 |
產(chǎn)品組分
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
18.75 |
0.25 |
|
L-Alanine |
89.0 |
4.45 |
0.049999997 |
|
L-Arginine hydrochloride |
211.0 |
147.5 |
0.69905216 |
|
L-Asparagine-H2O |
150.0 |
7.5 |
0.05 |
|
L-Aspartic acid |
133.0 |
6.65 |
0.05 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
17.56 |
0.09977272 |
|
L-Cystine 2HCl |
313.0 |
31.29 |
0.09996805 |
|
L-Glutamic Acid |
147.0 |
7.35 |
0.05 |
|
L-Histidine hydrochloride-H2O |
210.0 |
31.48 |
0.14990476 |
|
L-Isoleucine |
131.0 |
54.47 |
0.41580153 |
|
L-Leucine |
131.0 |
59.05 |
0.45076334 |
|
L-Lysine hydrochloride |
183.0 |
91.25 |
0.4986339 |
|
L-Methionine |
149.0 |
17.24 |
0.11570469 |
|
L-Phenylalanine |
165.0 |
35.48 |
0.2150303 |
|
L-Proline |
115.0 |
17.25 |
0.15 |
|
L-Serine |
105.0 |
26.25 |
0.25 |
|
L-Threonine |
119.0 |
53.45 |
0.44915968 |
|
L-Tryptophan |
204.0 |
9.02 |
0.04421569 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
55.79 |
0.21375479 |
|
L-Valine |
117.0 |
52.85 |
0.4517094 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.0035 |
1.4344263E-5 |
|
Choline chloride |
140.0 |
8.98 |
0.06414285 |
|
D-Calcium pantothenate |
477.0 |
2.24 |
0.0046960167 |
|
Folic Acid |
441.0 |
2.65 |
0.0060090707 |
|
Niacinamide |
122.0 |
2.02 |
0.016557377 |
|
Pyridoxine hydrochloride |
206.0 |
2.013 |
0.009771844 |
|
Riboflavin |
376.0 |
0.219 |
5.824468E-4 |
|
Thiamine hydrochloride |
337.0 |
2.17 |
0.0064391694 |
|
Vitamin B12 |
1355.0 |
0.68 |
5.0184503E-4 |
|
i-Inositol |
180.0 |
12.6 |
0.07 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhydrous) |
111.0 |
116.6 |
1.0504504 |
|
Cupric sulfate (CuSO4-5H2O) |
250.0 |
0.0013 |
5.2E-6 |
|
Ferric Nitrate (Fe(NO3)3-9H2O) |
404.0 |
0.05 |
1.2376238E-4 |
|
Ferric sulfate (FeSO4-7H2O) |
278.0 |
0.417 |
0.0015 |
|
Magnesium Chloride (anhydrous) |
95.0 |
28.64 |
0.30147368 |
|
Magnesium Sulfate (MgSO4) (anhydrous) |
120.0 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75.0 |
311.8 |
4.1573334 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
1200 |
14.285714 |
|
Sodium Chloride (NaCl) |
58.0 |
6995.5 |
120.61207 |
|
Sodium Phosphate dibasic (Na2HPO4)(anhydrous) |
142.0 |
71.02 |
0.50014085 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
62.5 |
0.45289856 |
|
Zinc sulfate (ZnSO4-7H2O) |
288.0 |
0.432 |
0.0015 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180.0 |
3151.0 |
17.505556 |
|
Hypoxanthine Na |
159.0 |
2.39 |
0.015031448 |
|
Linoleic Acid |
280.0 |
0.042 |
1.4999999E-4 |
|
Lipoic Acid |
206.0 |
0.105 |
5.097087E-4 |
|
Phenol Red |
376.4 |
8.1 |
0.021519661 |
|
Putrescine 2HCl |
161.0 |
0.081 |
5.031056E-4 |
|
Sodium Pyruvate |
110.0 |
55.0 |
0.5 |
|
Thymidine |
242.0 |
0.365 |
0.0015082645 |
|
L-Glutamine |
146 |
365 |
2.5 |
|
HEPES |
238 |
3574.5 |
15.018908 |
儲存條件
2～8℃避光保存，有效期1年。
注意事項
- 為了您的安全和健康，請穿實驗服并戴一次性手套操作。
- 本產(chǎn)品僅作科研用途！
Ver.CN20241014
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