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      Recombinant Rat IFN-gamma Protein

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻

      Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN-γ exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-γ, 59%64% with bovine, canine, equine, feline, and porcine IFN-γ, and 37%43% with cotton rat, mouse, and rat IFN-γ. Interferons have had increasing clinical usage in the treatment of a variety of disorders, at times being used in combination with chemo‐ or radiotherapy. IFN-γ increases the potential immunogenicity of vascular endothelial cells by up-regulation of intercellular adhesion molecule-1 (ICAM-1) and class I MHC antigen expression and by induction of class II MHC antigens and certain chemokines.

       

      Product Properties

      Synonyms

      Interferon-gamma, IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma.

      Accession

      P01581

      GeneID

      25712

      Source

      E.coli-derived rat IFN-γ protein, Gln23-Cys156.

      Molecular Weight

      Approximately 15.5 kDa.

      AA Sequence

      QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 97% by SDS-PAGE and HPLC analyses.

      Biological Activity

      The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard.

      Endotoxin

      0.1 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5% Trehalose and 0.05% Tween-80.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

      1 month, 2 to 8 °C under sterile conditions after reconstitution.

      3 months, -20 °C under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

      HB221025

      400-6111-883