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      Recombinant Mouse SDF-1α/CXCL12α Protein

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      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

      CXCL12 is belonging to the CXC chemokine family. SDF1 alpha  and SDF1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 is highly conserved between species, mouse CXCL12α shares approximately 93% amino acid sequence identity with human CXCL12α. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail.

       

      Product Properties

      Synonyms

      CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha,  SDF1, SDF1a, SDF1B, TLSF, TPAR1

      Accession

      P40224

      GeneID

      20315

      Source

      E.coli-derived mouse SDF-1α/CXCL12α protein, Lys22-Lys89.

      Molecular Weight

      Approximately 8.0 kDa.

      AA Sequence

      KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      >97% by SDS-PAGE and HPLC analyses.

      Biological Activity

      The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard.

      Endotoxin

      < 1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20 for 1 year.

      1 month, 2 to 8 °C under sterile conditions after reconstitution.
      3 months, -20 °C under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

       

      HB230104

       

      400-6111-883