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      Recombinant Rat SDF-1α/CXCL12α Protein

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      產(chǎn)品說明書

      FAQ

      COA

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      CXCL12 is belonging to the CXC chemokine family. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α. SDF-1 is highly conserved between species, rat CXCL12α shares approximately 96% amino acid sequence identity with human CXCL12α.

       

      Product Properties

      Synonyms

      CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha,  SDF1, SDF1a, SDF1B

      Accession

      Q9QZD1

      GeneID

      24772

      Source

      E.coli-derived rat SDF-1α/CXCL12α protein, Lys22-Lys89.

      Molecular Weight

      Approximately 7.9 kDa.

      AA Sequence

      KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

      Tag

      None

      Physical Appearance

      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      >97% by SDS-PAGE and HPLC analyses.

      Biological Activity

      The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard.

      Endotoxin

      < 1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20 for 1 year.

      1 month, 2 to 8 °C under sterile conditions after reconstitution.

      3 months, -20 °C under sterile conditions after reconstitution.

      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1.For your safety and health, please wear lab coats and disposable gloves for operation.

      2.For research use only!

      HB221111

      400-6111-883