Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

 

產(chǎn)品性質(zhì)

別名（Synonyms）	CD122; IL-15 R beta; IL-2 R beta; IL-2R subunit beta; IL2RB; P70-75; p75; RP5-1170K4.6; IL-15 R β; IL-2 R β; IL-2R subunit β
Uniprot No.	NP_000869
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）	Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala27-Asp239.
分子量(Molecular Weight)	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
外觀(Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
純度（Purity）	> 95% as determined by SDS-PAGE and HPLC
活性（Activity）	1.SPR Data：Human IL-2 R beta, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 0.25μM as determined in SPR assa 2.SPR Data: Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay.
內(nèi)毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制劑（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
復(fù)溶（Reconstitution）	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運(yùn)輸與保存方法

冰袋運(yùn)輸。-20℃至-80℃保存，一年有效期。

復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個(gè)月。復(fù)溶后，2-8°C 保存 2-7 天。

建議第一次使用時(shí)分裝凍存，避免反復(fù)凍融。

 

注意事項(xiàng)

1. 避免反復(fù)凍融。

2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

3. 本產(chǎn)品僅作科研用途！

 

產(chǎn)品數(shù)據(jù)

Tris-Bis PAGE

 

 

HB220624