表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ala27-Asp239.[Accession | NP_000869]

分子量大?。?/font>Molecular Weight）

The protein has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

內(nèi)毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

純度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

SPR Data: Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15, No Tag with an affinity constant of 2.88 nM as determined in SPR assay 

SPR Data: Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.216 μM as determined in SPR assay .

制劑（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重構(gòu)方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.