Recombinant Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein,His-Avi Tag
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表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source） |
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.. It containsGly17-Gln208(F176).Uniprot | P08637-1 |
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分子量大小 （Molecular Weight） |
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result. |
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內(nèi)毒素（Endotoxin） |
Less than 1EU per μg by the LAL method. |
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純度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
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活性（Activity） |
ELISA Data: Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA SPR Data:Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay |
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制劑（Formulation） |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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重構(gòu)方法（Reconstitution） |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
儲存條件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事項
1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
Ver. CN20230602
