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rProtein A/G Agarose Resin 蛋白A/G瓊脂糖純化樹脂

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產(chǎn)品簡介

天然蛋白A（Protein A）是一種發(fā)現(xiàn)于金黃色葡萄球菌的細胞壁表面蛋白，天然蛋白G（Protein G）是一種分離自G型或C型鏈球菌屬的細胞表面蛋白，二者功能相似，主要通過與免疫球蛋白（Ig）的Fc區(qū)相互作用，可結(jié)合大多數(shù)哺乳動物的IgG。然而兩者結(jié)合特異性上有所不同（詳細見附錄1，蛋白A，G對不同物種Ig的結(jié)合能力總表）。蛋白A，蛋白G常共價偶聯(lián)在固體載體如瓊脂糖珠或丙烯酸樹脂小珠上，直接用以做免疫沉淀（IP）或者免疫共沉淀（Co-IP）來進行蛋白功能相關(guān)研究，或者直接裝在層析柱上來純化單克隆或者多克隆抗體。

本品使用的是基因改造后的蛋白A和蛋白G，不僅維持其本身的Ig親和特性，同時也去除了天然蛋白本身的非主要結(jié)合域以降低非特異性結(jié)合。本品同時將蛋白A和蛋白G共價偶聯(lián)到瓊脂糖凝膠微球表面，比單獨的蛋白A或者蛋白G都有更廣的結(jié)合范圍，適合于所有蛋白A瓊脂糖珠和蛋白G瓊脂糖珠單獨可以結(jié)合的Ig，實用性更高。

翌圣為您提供蛋白純化實驗整體解決方案，相關(guān)產(chǎn)品選購請參考：蛋白純化系列產(chǎn)品-選購指南

產(chǎn)品信息

貨號	36403ES03 / 36403ES05/36403ES08/36403ES25/36403ES60
規(guī)格	1 mL /2 mL/5 mL/25 mL/100 mL

產(chǎn)品性質(zhì)

基質(zhì)（Matrix）	高度交聯(lián)的4%瓊脂糖微球
配體（Ligand）	重組蛋白A/G
孔徑（Bead size）	45-165 µm
最大流速（Flow_max）	0.3 MPa, 3 bar
儲存緩沖液（Buffer）	含20%乙醇的1×PBS
載量（Capacity）	約10-15 mg Rabbit IgG/mL 基質(zhì)
pH范圍（pH range）	3-10

儲存條件

2~8℃保存，有效期2年。

附錄1. 蛋白A，G對不同物種Ig的結(jié)合能力總表

免疫球蛋白亞型	Protein A	Protein G	免疫球蛋白亞型	Protein A	Protein G
Human IgG	++++	++++	Mouse IgG	++++	++++
Human IgG1	++++	++++	Mouse IgG1	+	++++
Human IgG2	++++	++++	Mouse IgG2a	++++	++++
Human IgG3	+	++++	Mouse IgG2b	+++	+++
Human IgG4	++++	++++	Mouse IgG3	++	+++
Human IgM	Use anti-Human IgM		Mouse IgM	Use anti-Mouse IgM
Human IgE	NR	NR	Chicken IgG (IgY)	NR	NR
Human IgA	+	NR	Cow IgG	++	++++
Human IgA1	+	NR	Goat IgG	+	++++
Human IgA2	+	NR	Goat IgG1	+	++++
Human IgD	Use anti-Human IgD		Goat IgG2	++++	++++
Rat IgG	+	++	Goat IgM	NR	NR
Rat IgG1	NR	+	Guinea Pig IgG	++++	++
Rat IgG2a	NR	NR	Guinea Pig IgG1	++++	++
Rat IgG2b	NR	+	Guinea Pig IgG2	++++	++
Rat IgG3	+	++	Hamster IgG	+	++
Sheep IgG	+	++	Horse IgG	+	++++
Sheep IgG1	+	++	Rabbit IgG	++++	+++
Sheep IgG2	+	++	Rabbit IgM	NR	NR
Sheep IgM	NR	NR	Rabbit All isotypes	+++	++
Pig IgG	+++	+++	Monkey IgG	++++	++++
Cat IgG	++++	+	Donkey IgG	++	++++
Dog IgG	++++	+
（+）= weak binding；（++）= moderate binding；（++++）= strong binding；NR= not recommended; （-）= not tested；

Ver.CN20230824

[1] Chen LJ, Zhang NN, Zhou CX, et al. Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation. Cell Death Differ. 2022;29(2):366-380. doi:10.1038/s41418-021-00861-5(IF:15.828)
[2] Zhang K, Chen S, Yang Q, et al. The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodents [published correction appears in Nat Commun. 2022 Jun 1;13(1):3164]. Nat Commun. 2022;13(1):1423. Published 2022 Mar 17. doi:10.1038/s41467-022-29068-z(IF:14.919)
[3] Zhu D, Chen C, Liu X, et al. Osteosarcoma cell proliferation suppression via SHP-2-mediated inactivation of the JAK/STAT3 pathway by tubocapsenolide A. J Adv Res. 2021;34:79-91. Published 2021 Jun 11. doi:10.1016/j.jare.2021.06.004(IF:10.479)
[4] Zheng J, Yao L, Zhou Y, et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021;12(10):871. Published 2021 Sep 24. doi:10.1038/s41419-021-04159-9(IF:8.469)
[5] Wang J, Zhao D, Ding CZ, et al. MicroRNA-194: a novel regulator of glucagon-like peptide-1 synthesis in intestinal L cells. Cell Death Dis. 2021;12(1):113. Published 2021 Jan 21. doi:10.1038/s41419-020-03366-0(IF:8.469)
[6] Zhu H, Guo Y, Huang A, et al. HDAC3-Regulated PGE2 Production by Microglia Induces Phobic Anxiety Susceptibility After Stroke and Pointedly Exploiting a Signal-Targeted Gamma Visual Stimulation New Therapy. Front Immunol. 2022;13:845678. Published 2022 Feb 18. doi:10.3389/fimmu.2022.845678(IF:7.561)
[7] Wang Z, Xu J, Feng J, et al. Structural and Functional Analyses of Type I IFNa Shed Light Into Its Interaction With Multiple Receptors in Fish. Front Immunol. 2022;13:862764. Published 2022 Mar 22. doi:10.3389/fimmu.2022.862764(IF:7.561)
[8] Ni H, Qin H, Sun C, et al. MiR-375 reduces the stemness of gastric cancer cells through triggering ferroptosis. Stem Cell Res Ther. 2021;12(1):325. Published 2021 Jun 5. doi:10.1186/s13287-021-02394-7(IF:6.832)
[9] Gao L, Guo Q, Li X, et al. MiR-873/PD-L1 axis regulates the stemness of breast cancer cells [published correction appears in EBioMedicine. 2019 Nov;49:389-390]. EBioMedicine. 2019;41:395-407. doi:10.1016/j.ebiom.2019.02.034(IF:6.680)
[10] Yang X, Li X, Zhong C, et al. Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2. Oxid Med Cell Longev. 2021;2021:3823122. Published 2021 Nov 8. doi:10.1155/2021/3823122(IF:6.543)
[11] Xie Y, Peng J, Pang J, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152(3):368-380. doi:10.1111/jnc.14926(IF:4.870)
[12] Sun Z, Zhang L, Li L, et al. Galectin-3 mediates cardiac remodeling caused by impaired glucose and lipid metabolism through inhibiting two pathways of activating Akt. Am J Physiol Heart Circ Physiol. 2021;320(1):H364-H380. doi:10.1152/ajpheart.00523.2020(IF:4.733)
[13] Zhao X, Huang W, Guo J, et al. PLAAT1 promotes p53 degradation via autophagy-lysosome pathway in zebrafish. Fish Shellfish Immunol. 2022;125:48-53. doi:10.1016/j.fsi.2022.05.001(IF:4.581)
[14] Gu H, Duan Z. Silencing of circDPP4 suppresses cell progression of human prostate cancer and enhances docetaxel cytotoxicity through regulating the miR-564/ZIC2 axis. J Gene Med. 2022;24(3):e3403. doi:10.1002/jgm.3403(IF:4.565)
[15] Zheng L, Zhang Z, Zhang S, et al. RNA Binding Protein RNPC1 Inhibits Breast Cancer Cell Metastasis via Activating STARD13-Correlated ceRNA Network. Mol Pharm. 2018;15(6):2123-2132. doi:10.1021/acs.molpharmaceut.7b01123(IF:4.556)
[16] Guo X, Xiang C, Zhang Z, Zhang F, Xi T, Zheng L. Displacement of Bax by BMF Mediates STARD13 3'UTR-Induced Breast Cancer Cells Apoptosis in an miRNA-Depedent Manner. Mol Pharm. 2018;15(1):63-71. doi:10.1021/acs.molpharmaceut.7b00727(IF:4.440)
[17] Meng P, Zhang YF, Zhang W, et al. Identification of the atypical cadherin FAT1 as a novel glypican-3 interacting protein in liver cancer cells. Sci Rep. 2021;11(1):40. Published 2021 Jan 8. doi:10.1038/s41598-020-79524-3(IF:4.380)
[18] Li J, Ye W, Xu W, et al. Activation of autophagy inhibits epithelial to mesenchymal transition process of human lens epithelial cells induced by high glucose conditions. Cell Signal. 2020;75:109768. doi:10.1016/j.cellsig.2020.109768(IF:3.968)
[19] Li H, Hu X, Cheng C, et al. Ribosome production factor 2 homolog promotes migration and invasion of colorectal cancer cells by inducing epithelial-mesenchymal transition via AKT/Gsk-3β signaling pathway [published online ahead of print, 2022 Jan 28]. Biochem Biophys Res Commun. 2022;597:52-57. doi:10.1016/j.bbrc.2022.01.090(IF:3.575)
[20] Zhang H, Lu Y, Wu B, Xia F. Semaphorin 3A mitigates lipopolysaccharide-induced chondrocyte inflammation, apoptosis and extracellular matrix degradation by binding to Neuropilin-1. Bioengineered. 2021;12(2):9641-9654. doi:10.1080/21655979.2021.1974806(IF:3.269)
[21] Zhao Q, Liu Y, Wang T, et al. MiR-375 inhibits the stemness of breast cancer cells by blocking the JAK2/STAT3 signaling. Eur J Pharmacol. 2020;884:173359. doi:10.1016/j.ejphar.2020.173359(IF:3.263)
[22] Sun Z, Wang Z, Li L, et al. RAGE/galectin-3 yields intraplaque calcification transformation via sortilin. Acta Diabetol. 2019;56(4):457-472. doi:10.1007/s00592-018-1273-1(IF:2.996)
[23] Zhang D, Chen X, Zheng D. A Novel MIR503HG/miR-497-5p/CCL19 Axis Regulates High Glucose-Induced Cell Apoptosis, Inflammation, and Fibrosis in Human HK-2 Cells. Appl Biochem Biotechnol. 2022;194(5):2061-2076. doi:10.1007/s12010-021-03776-6(IF:2.926)
[24] Zhang F, Ni H, Li X, Liu H, Xi T, Zheng L. LncRNA FENDRR attenuates adriamycin resistance via suppressing MDR1 expression through sponging HuR and miR-184 in chronic myelogenous leukaemia cells. FEBS Lett. 2019;593(15):1993-2007. doi:10.1002/1873-3468.13480(IF:2.675)
[25] Pan T, Qian Y, Li T, et al. Acetyl l-carnitine protects adipose-derived stem cells against serum-starvation: regulation on the network composed of reactive oxygen species, autophagy, apoptosis and senescence. Cytotechnology. 2022;74(1):105-121. doi:10.1007/s10616-021-00514-y(IF:2.058)
[26] Li X, Zhang N, Zhang Y, et al. E3 ligase Fbw7 participates in oxidative stress?induced myocardial cell injury via interacting with Mcl?1. Mol Med Rep. 2019;20(2):1561-1568. doi:10.3892/mmr.2019.10394(IF:1.851)

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