成人欧美一区二区三区,天天干天天操天天爱,免费特黄一级欧美大片在线看 ,亚洲中文字幕无码一区日日添

    <span id="alen4"></span>

    <li id="alen4"></li>

      Recombinant Human R spondin 3/RSPO3 Protein,His Tag

      更多活動(dòng)更多優(yōu)惠,盡在翌圣商城》

      批量采購(gòu), 請(qǐng)點(diǎn)擊詢價(jià)

      產(chǎn)品說明書

      FAQ

      COA

      已發(fā)表文獻(xiàn)

      產(chǎn)品描述

       

      1. Spondin(Rspo)蛋白屬于Wnt調(diào)節(jié)劑的Rspo家族。目前,該家族由四個(gè)結(jié)構(gòu)相關(guān)的分泌配體(Rspo 1-4)組成,所有配體都含有弗林蛋白酶樣和凝血酶敏感蛋白結(jié)構(gòu)域。Rspo蛋白可以與卷曲/LRP6受體復(fù)合物相互作用,導(dǎo)致細(xì)胞內(nèi)信號(hào)蛋白β-catenin的穩(wěn)定和積累。這種活性有效地激活并增加了β-catenin的后續(xù)核信號(hào)傳導(dǎo)。R-Spondin還可以與G蛋白偶聯(lián)受體LGR-4和LGR-5結(jié)合。Rspo/β-catenin信號(hào)傳導(dǎo)可以作為轉(zhuǎn)化表型的誘導(dǎo)劑,也可以調(diào)節(jié)某些干細(xì)胞群的增殖和分化。

      本品重組人R-Spondin-3蛋白,以凍干粉形式提供,高活性,高純度。

       

      性能參數(shù)

       

      表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source)

      Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1]

      分子量大小(Molecular Weight)

      The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.

      內(nèi)毒素(Endotoxin)

      <1EU per μg by the LAL method.

      純度(Purity)

      > 95% as determined by Tris-Bis PAGE

       

       

                   活性(Activity)

      ELISA Data: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA 

      SPR Data: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200)

      制劑(Formulation)

      Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

      外觀(Physical Appearance)

      白色凍干粉

      標(biāo)簽(Tag)

      C-His

       

      儲(chǔ)存條件

       

      -25~-15℃保存,收到貨之后有效期1年。

      復(fù)溶后, 2 ~8 °C保存,7天有效期。復(fù)溶后, -85~-65℃保存3個(gè)月有效期。

       

      復(fù)溶方法

       

      開蓋之前,先離心。在無菌蒸餾水中重新配制至0.1-1.0 mg/mL的濃度,可根據(jù)后續(xù)實(shí)驗(yàn)更進(jìn)一步稀釋。避免反復(fù)凍融。

       

      注意事項(xiàng)

       

      1. 為了您的安全和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
      2. 本產(chǎn)品僅作科研用途。

             

      Ver. CN20240812

       

       

      400-6111-883