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產(chǎn)品描述
本品重組人R-Spondin-3蛋白,以凍干粉形式提供,高活性,高純度。
性能參數(shù)
表達(dá)區(qū)間及表達(dá)系統(tǒng)(Source) |
Human R spondin 3/RSPO3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln22-Val146.[Accession | Q9BXY4-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result. |
內(nèi)毒素(Endotoxin) |
<1EU per μg by the LAL method. |
純度(Purity) |
> 95% as determined by Tris-Bis PAGE |
活性(Activity) |
ELISA Data: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA SPR Data: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) |
制劑(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
外觀(Physical Appearance) |
白色凍干粉 |
標(biāo)簽(Tag) |
C-His |
儲(chǔ)存條件
-25~-15℃保存,收到貨之后有效期1年。
復(fù)溶后, 2 ~8 °C保存,7天有效期。復(fù)溶后, -85~-65℃保存,3個(gè)月有效期。
復(fù)溶方法
開蓋之前,先離心。在無菌蒸餾水中重新配制至0.1-1.0 mg/mL的濃度,可根據(jù)后續(xù)實(shí)驗(yàn)更進(jìn)一步稀釋。避免反復(fù)凍融。
注意事項(xiàng)
Ver. CN20240812