IF>5000經(jīng)典預(yù)混液Hieff? qPCR SYBR Green Master Mix(No Rox)
批量采購， 請點(diǎn)擊詢價
產(chǎn)品說明書
FAQ
COA
已發(fā)表文獻(xiàn)
產(chǎn)品描述
Hieff® qPCR SYBR Green Master Mix(No Rox)是2×實(shí)時定量PCR擴(kuò)增的預(yù)混合溶液。Mix中含有熱啟動Hieff® DNA Polymerase、SYBR Green I、dNTPs、Mg2+。使用時，僅需在擴(kuò)增體系中加入模板和引物即可進(jìn)行實(shí)時熒光定量PCR，大大簡化操作過程，降低污染幾率。
本品采用的DNA聚合酶配體可以隨溫度變化實(shí)時調(diào)節(jié)DNA聚合酶活性。配方添加了有效抑制非特異性PCR擴(kuò)增的因子和提升PCR反應(yīng)擴(kuò)增效率的因子，使定量PCR可以在寬廣的定量區(qū)域內(nèi)獲得良好的線性關(guān)系。
適用機(jī)型
Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;
Eppendorf: Mastercycler ep realplex, realplex 2 s;
Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;
Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;
Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR.
運(yùn)輸和保存方式
冰袋運(yùn)輸。-20℃避光儲存，有效期18個月。
本品避免反復(fù)凍融。產(chǎn)品中含有熒光染料SYBR Green I，保存或配制反應(yīng)體系時需避免強(qiáng)光照射。
注意事項(xiàng)
1. 推薦使用本公司cDNA合成試劑盒（貨號：11123ES），以有效去除RNA樣品中殘留的基因組。
2. 解凍后Master Mix可能出現(xiàn)絮狀物質(zhì)，4℃放置并上下顛倒混勻至溶液澄清，不影響試劑性能。
3. 為了您的安全和健康，請穿實(shí)驗(yàn)服并佩戴一次性手套操作。
4. 本產(chǎn)品僅作科研用途！
反應(yīng)體系（推薦冰上配制）
|
組分 |
體積（μL） |
體積（μL） |
終濃度 |
|
Hieff® qPCR SYBR Green Master Mix (No Rox) |
25 |
10 |
1× |
|
Forward Primer (10 μM) |
1 |
0.4 |
0.2 μM |
|
Reverse Primer (10 μM) |
1 |
0.4 |
0.2 μM |
|
模板DNA |
X |
X |
- |
|
無菌超純水 |
to 50 |
to 20 |
- |
【注】： 使用前務(wù)必充分混勻，避免劇烈震蕩產(chǎn)生過多氣泡。
a) 引物濃度：通常引物終濃度為0.2 μM，也可以根據(jù)情況在0.1-1.0 μM之間進(jìn)行調(diào)整。
b) 模板濃度：如模板類型為未稀釋cDNA原液，使用體積不應(yīng)超過qPCR反應(yīng)總體積的1/10。
c) 模板稀釋：cDNA原液建議5-10倍稀釋，最佳模板加入量以擴(kuò)增得到的CT值在20-30個循環(huán)為好。
d) 反應(yīng)體系：推薦使用20 μL或50 μL，以保證目的基因擴(kuò)增的有效性和重復(fù)性。
e) 體系配制：請于超凈工作臺內(nèi)配制，并使用無核酸酶殘留的槍頭、反應(yīng)管；推薦使用帶濾芯的槍頭。避免交叉污染和氣溶膠污染。
【注】: 高特異性可選擇兩步法，高效率擴(kuò)增可選擇三步法。
a) 預(yù)變性時間：根據(jù)不同模板和引物的具體情況可適當(dāng)縮短至2 min。
b) 退火溫度和時間：請根據(jù)引物和目的基因的長度進(jìn)行調(diào)整。
c) 熒光信號采集（★）：請按照儀器使用說明書要求進(jìn)行實(shí)驗(yàn)程序設(shè)置，幾種常見儀器的時間設(shè)定如下：
30sec以上：Applied Biosystems: StepOne, StepOne Plus, 7500 Fast；Roche Applied Science: LightCycler 480；Bio-Rad: CFX96
31sec以上：Applied Biosystems: 7300
34sec以上：Applied Biosystems: 7500
d) 熔解曲線：通常情況下可以使用儀器默認(rèn)程序。
結(jié)果分析
定量實(shí)驗(yàn)至少需要三個生物學(xué)重復(fù)。反應(yīng)結(jié)束后需要確認(rèn)擴(kuò)增曲線及熔解曲線。
1) 擴(kuò)增曲線：標(biāo)準(zhǔn)擴(kuò)增曲線為S型。
Ct值落在20-30之間時，定量分析最準(zhǔn)確；
Ct值小于10時，需要將稀釋模板后，重新進(jìn)行實(shí)驗(yàn)；
Ct值介于30-35之間時，需要提高模板濃度，或者增大反應(yīng)體系的體積，以提高擴(kuò)增效率，保證結(jié)果分析的準(zhǔn)確性；
Ct值大于35時，檢測結(jié)果無法定量分析基因的表達(dá)量，但可用于定性分析。
2) 熔解曲線：
熔解曲線單峰，表明反應(yīng)特異性好可以進(jìn)行定量結(jié)果分析；若熔解曲線出現(xiàn)雙峰或者多峰，則不能進(jìn)行定量分析。
熔解曲線出現(xiàn)雙峰，需要通過DNA瓊脂糖凝膠電泳判斷非目標(biāo)峰是引物二聚體還是非特異性擴(kuò)增。
如果是引物二聚體，建議降低引物濃度，或者重新設(shè)計(jì)擴(kuò)增效率高的引物。
如果是非特異性擴(kuò)增，請?zhí)岣咄嘶饻囟?，或者重新設(shè)計(jì)更高特異性的引物。
引物設(shè)計(jì)指南
1. 推薦引物長度25 bp左右。擴(kuò)增產(chǎn)物長度150 bp為佳，可以在100 bp-300 bp內(nèi)選擇。
2. 正向引物和反向引物的Tm值相差不宜超過2℃。引物Tm值60℃-65℃為佳。
3. 引物堿基分布要均勻，避免出現(xiàn)連續(xù)的4個相同堿基，GC含量控制在50%左右。3’端最后一個堿基最好為G或C。
4. 引物內(nèi)部或者正反兩條引物間最好避免出現(xiàn)有3個堿基以上的互補(bǔ)序列。
5. 引物特異性需要用NCBI BLAST程序進(jìn)行核對。避免引物3’端有2個堿基以上的非特異性互補(bǔ)。
6. 設(shè)計(jì)完成的引物需要進(jìn)行擴(kuò)增效率的檢測，只有具備相同擴(kuò)增效率的引物才可用于定量比較分析。
相關(guān)產(chǎn)品
|
產(chǎn)品名稱 |
貨號 |
規(guī)格 |
|
11119ES60 |
100 T |
|
|
Hifair® II 1st Strand cDNA Synthesis Kit (gDNA digester plus) |
11121ES60 |
100 T |
|
Hifair® II 1st Strand cDNA Synthesis SuperMix for qPCR(gDNA digester plus) HOT |
11123ES60 |
100 T |
|
11201ES08 |
5 ml |
|
|
11202ES08 |
5 ml |
|
|
11203ES08 |
5 ml |
HB210720
Q：不加 ROX 的qPCR mix 后續(xù)需換用其他儀器（需要 ROX），是否可以單獨(dú)加 ROX后再使用？
A：可以。
Q：可以用于microRNA 或lncRNA 等非編碼RNA 嗎？環(huán)狀 RNA 呢？
A：不推薦。
Q：建議qPCR 實(shí)驗(yàn)用幾步法？
A：常用 2 步法。需提高擴(kuò)增特異性，可選用 2 步法或提高退火溫度。在擴(kuò)增效率低， ct 值過大的時候，可以改用 3 步法或延長延伸時間。
Q：預(yù)變性 5 min 調(diào)整成了 10min，對于實(shí)驗(yàn)結(jié)果有影響嗎？
A：有影響，預(yù)變性時間較長可能會影響酶的活性，導(dǎo)致 PCR 產(chǎn)物的產(chǎn)量有所降低。
Q：qPCR 實(shí)驗(yàn)結(jié)果的有效性？為什么建議Ct 值要大于 15？
A：有效性要滿足三個條件：（1）標(biāo)準(zhǔn)曲線：擴(kuò)增效率范圍:90-110%，對應(yīng)斜率為 -3--3.5。 R2＞0.98。 (擴(kuò)增效率=10-1/斜率-1)，當(dāng)斜率=-3.32 時，擴(kuò)增效率=100%。（2）擴(kuò) 增曲線：S 型曲線，且 Ct 值在 15－35 之間，陰性對照 Ct＞35 或無 Ct 值。（3）熔解曲線：為單一峰。
Ct 值大于 15 個循環(huán)是因?yàn)?/font> 3-15 個循環(huán)的熒光值標(biāo)準(zhǔn)差的 10 倍是熒光閾值，Ct 值太小了會影響曲線。
Q：同一基因復(fù)孔間熔解曲線 Tm 值有差異？
A：同樣的擴(kuò)增產(chǎn)物也會出現(xiàn)Tm 值有微小差異，一般差異在 1 度以內(nèi)都可以接受。
Q：為什么稀釋了模板CT 值反而變小了？
A：一般 CT 值與模板起始濃度呈負(fù)相關(guān)，濃度越高，CT 值越小。但也有很多特殊情況， 比如體系中存在抑制物或是模板不純，這時候稀釋模板反而能使 CT 值變低。
Q：11201,11202,11203區(qū)別是什么呢？如何選擇呢？
A：里面預(yù)混的ROX濃度不同，需要根據(jù)儀器的型號進(jìn)行對應(yīng)選擇。
[1] Medina-Puche L, Tan H, Dogra V, et al. A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens. Cell. 2020;182(5):1109-1124.e25. doi:10.1016/j.cell.2020.07.020(IF:38.637)
[2] Wan S, Ni L, Zhao X, et al. Costimulation molecules differentially regulate the ERK-Zfp831 axis to shape T follicular helper cell differentiation. Immunity. 2021;54(12):2740-2755.e6. doi:10.1016/j.immuni.2021.09.018(IF:31.745)
[3] Han X, Wang R, Zhou Y, et al. Mapping the Mouse Cell Atlas by Microwell-Seq [published correction appears in Cell. 2018 May 17;173(5):1307]. Cell. 2018;172(5):1091-1107.e17. doi:10.1016/j.cell.2018.02.001(IF:31.398)
[4] Tang Y, Fang G, Guo F, et al. Selective Inhibition of STRN3-Containing PP2A Phosphatase Restores Hippo Tumor-Suppressor Activity in Gastric Cancer. Cancer Cell. 2020;38(1):115-128.e9. doi:10.1016/j.ccell.2020.05.019(IF:26.602)
[5] Chang D, Xing Q, Su Y, et al. The Conserved Non-coding Sequences CNS6 and CNS9 Control Cytokine-Induced Rorc Transcription during T Helper 17 Cell Differentiation. Immunity. 2020;53(3):614-626.e4. doi:10.1016/j.immuni.2020.07.012(IF:22.553)
[6] Wang X, Ni L, Wan S, et al. Febrile Temperature Critically Controls the Differentiation and Pathogenicity of T Helper 17 Cells. Immunity. 2020;52(2):328-341.e5. doi:10.1016/j.immuni.2020.01.006(IF:22.553)
[7] Xiao J, Li W, Zheng X, et al. Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection. Immunity. 2020;52(1):109-122.e6. doi:10.1016/j.immuni.2019.11.015(IF:21.522)
[8] Zhao X, Di Q, Yu J, et al. USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy. Autophagy. 2022;18(4):891-908. doi:10.1080/15548627.2021.1963155(IF:16.016)
[9] Sun Z, Tang Y, Zhang Y, et al. Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division. Nat Commun. 2021;12(1):5941. Published 2021 Oct 12. doi:10.1038/s41467-021-26203-0(IF:14.919)
[10] Meng YM, Jiang X, Zhao X, et al. Hexokinase 2-driven glycolysis in pericytes activates their contractility leading to tumor blood vessel abnormalities. Nat Commun. 2021;12(1):6011. Published 2021 Oct 14. doi:10.1038/s41467-021-26259-y(IF:14.919)
[11] Wang P, Geng J, Gao J, et al. Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis. Nat Commun. 2019;10(1):755. Published 2019 Feb 14. doi:10.1038/s41467-019-08680-6(IF:11.878)
[12] Zhao X, Di Q, Liu H, et al. MEF2C promotes M1 macrophage polarization and Th1 responses. Cell Mol Immunol. 2022;19(4):540-553. doi:10.1038/s41423-022-00841-w(IF:11.530)
[13] Zhang J, Li Y, Liu H, et al. Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis. J Exp Clin Cancer Res. 2022;41(1):24. Published 2022 Jan 15. doi:10.1186/s13046-022-02242-3(IF:11.161)
[14] Zhang M, Liu F, Zhou P, et al. The MTOR signaling pathway regulates macrophage differentiation from mouse myeloid progenitors by inhibiting autophagy. Autophagy. 2019;15(7):1150-1162. doi:10.1080/15548627.2019.1578040(IF:11.059)
[15] Wang X, Chen K, Zhou M, et al. GmNAC181 promotes symbiotic nodulation and salt tolerance of nodulation by directly regulating GmNINa expression in soybean [published online ahead of print, 2022 Jun 25]. New Phytol. 2022;10.1111/nph.18343. doi:10.1111/nph.18343(IF:10.152)
[16] Fan T, Wang S, Jiang Z, et al. Controllable assembly of skeletal muscle-like bundles through 3D bioprinting. Biofabrication. 2021;14(1):10.1088/1758-5090/ac3aca. Published 2021 Dec 1. doi:10.1088/1758-5090/ac3aca(IF:10.020)
[17] Wu F, Duan Z, Xu P, et al. Genome and systems biology of Melilotus albus provides insights into coumarins biosynthesis. Plant Biotechnol J. 2022;20(3):592-609. doi:10.1111/pbi.13742(IF:9.803)
[18] Wu F, Zhang C, Zhao C, et al. Prostaglandin E1 Inhibits GLI2 Amplification-Associated Activation of the Hedgehog Pathway and Drug Refractory Tumor Growth. Cancer Res. 2020;80(13):2818-2832. doi:10.1158/0008-5472.CAN-19-2052(IF:9.727)
[19] Chen K, Gao J, Sun S, et al. BONZAI Proteins Control Global Osmotic Stress Responses in Plants. Curr Biol. 2020;30(24):4815-4825.e4. doi:10.1016/j.cub.2020.09.016(IF:9.601)
[20] Luo F, Yang W, Yin M, et al. A chromosome-level genome of the human blood fluke Schistosoma japonicum identifies the genomic basis of host-switching. Cell Rep. 2022;39(1):110638. doi:10.1016/j.celrep.2022.110638(IF:9.423)
[21] Xiang H, Tao Y, Jiang Z, et al. Vps33B controls Treg cell suppressive function through inhibiting lysosomal nutrient sensing complex-mediated mTORC1 activation. Cell Rep. 2022;39(11):110943. doi:10.1016/j.celrep.2022.110943(IF:9.423)
[22] Yu L, Chu C, Yuan Y, et al. Activity in projection neurons from prelimbic cortex to the PVT is necessary for retrieval of morphine withdrawal memory. Cell Rep. 2021;35(1):108958. doi:10.1016/j.celrep.2021.108958(IF:9.423)
[23] Zhu L, He S, Huang L, et al. Chaperone-mediated autophagy degrades Keap1 and promotes Nrf2-mediated antioxidative response. Aging Cell. 2022;21(6):e13616. doi:10.1111/acel.13616(IF:9.304)
[24] Xiang G, Wang S, Chen L, et al. UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer. Cell Death Dis. 2022;13(5):451. Published 2022 May 12. doi:10.1038/s41419-022-04914-6(IF:8.469)
[25] Liu H, Xing R, Ou Z, et al. G-protein-coupled receptor GPR17 inhibits glioma development by increasing polycomb repressive complex 1-mediated ROS production. Cell Death Dis. 2021;12(6):610. Published 2021 Jun 12. doi:10.1038/s41419-021-03897-0(IF:8.469)
[26] He G, Cao Y, Wang J, et al. WUSCHEL-Related Homeobox Genes Cooperate with Cytokinin to Promote Bulbil Formation in Lilium lancifolium [published online ahead of print, 2022 Jun 7]. Plant Physiol. 2022;kiac259. doi:10.1093/plphys/kiac259(IF:8.340)
[27] Chen D, Li J, Huang Y, et al. Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3. J Neuroinflammation. 2022;19(1):112. Published 2022 May 16. doi:10.1186/s12974-022-02471-5(IF:8.322)
[28] Wei P, Wang K, Luo C, et al. Cordycepin confers long-term neuroprotection via inhibiting neutrophil infiltration and neuroinflammation after traumatic brain injury. J Neuroinflammation. 2021;18(1):137. Published 2021 Jun 15. doi:10.1186/s12974-021-02188-x(IF:8.322)
[29] Zhou M, Xu W, Wang J, et al. Boosting mTOR-dependent autophagy via upstream TLR4-MyD88-MAPK signalling and downstream NF-κB pathway quenches intestinal inflammation and oxidative stress injury. EBioMedicine. 2018;35:345-360. doi:10.1016/j.ebiom.2018.08.035(IF:8.143)
[30] Zhou M, Xu W, Wang J, et al. Boosting mTOR-dependent autophagy via upstream TLR4-MyD88-MAPK signalling and downstream NF-κB pathway quenches intestinal inflammation and oxidative stress injury. EBioMedicine. 2018;35:345-360. doi:10.1016/j.ebiom.2018.08.035(IF:8.143)
[31] Wang Q, Zhou X, Yang L, et al. The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis [published correction appears in Cell Rep. 2020 Nov 24;33(8):108442]. Cell Rep. 2020;32(11):108158. doi:10.1016/j.celrep.2020.108158(IF:8.109)
[32] Jin M, Wang Y, An X, et al. Phenotypic and transcriptomic changes in the corneal epithelium following exposure to cigarette smoke. Environ Pollut. 2021;287:117540. doi:10.1016/j.envpol.2021.117540(IF:8.071)
[33] Zhang Y, Xu J, Liu S, et al. Embryonic stem cell-derived extracellular vesicles enhance the therapeutic effect of mesenchymal stem cells. Theranostics. 2019;9(23):6976-6990. Published 2019 Sep 21. doi:10.7150/thno.35305(IF:8.063)
[34] He S, Huang L, Shao C, et al. Several miRNAs derived from serum extracellular vesicles are potential biomarkers for early diagnosis and progression of Parkinson's disease. Transl Neurodegener. 2021;10(1):25. Published 2021 Jul 28. doi:10.1186/s40035-021-00249-y(IF:8.014)
[35] Ou Z, Ma Y, Sun Y, et al. A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism. Cell Rep. 2019;26(11):2984-2997.e4. doi:10.1016/j.celrep.2019.02.060(IF:7.815)
[36] Chen Y, Yi X, Huo B, et al. BRD4770 functions as a novel ferroptosis inhibitor to protect against aortic dissection. Pharmacol Res. 2022;177:106122. doi:10.1016/j.phrs.2022.106122(IF:7.658)
[37] Wu Z, Lu Z, Li L, et al. Identification and Validation of Ferroptosis-Related LncRNA Signatures as a Novel Prognostic Model for Colon Cancer. Front Immunol. 2022;12:783362. Published 2022 Jan 26. doi:10.3389/fimmu.2021.783362(IF:7.561)
[38] Liu Y, Jin J, Xu H, et al. Construction of a pH-responsive, ultralow-dose triptolide nanomedicine for safe rheumatoid arthritis therapy. Acta Biomater. 2021;121:541-553. doi:10.1016/j.actbio.2020.11.027(IF:7.242)
[39] Zhou M, Li B, Liu J, Hong L. Genomic, Immunological, and Clinical Characterization of Pyroptosis in Ovarian Cancer. J Inflamm Res. 2021;14:7341-7358. Published 2021 Dec 24. doi:10.2147/JIR.S344554(IF:6.922)
[40] Yu L, Liu S, Wang C, et al. Embryonic stem cell-derived extracellular vesicles promote the recovery of kidney injury. Stem Cell Res Ther. 2021;12(1):379. Published 2021 Jul 2. doi:10.1186/s13287-021-02460-0(IF:6.832)
[41] Zhao S, Zhang C, Xu J, et al. Dppa3 facilitates self-renewal of embryonic stem cells by stabilization of pluripotent factors. Stem Cell Res Ther. 2022;13(1):169. Published 2022 Apr 27. doi:10.1186/s13287-022-02846-8(IF:6.832)
[42] Yuan J, Jiang X, Lan H, et al. Multi-Omics Analysis of the Therapeutic Value of MAL2 Based on Data Mining in Human Cancers. Front Cell Dev Biol. 2022;9:736649. Published 2022 Jan 17. doi:10.3389/fcell.2021.736649(IF:6.684)
[43] Zhang Q, Zhang Y, Zhang J, et al. p66α Suppresses Breast Cancer Cell Growth and Migration by Acting as Co-Activator of p53. Cells. 2021;10(12):3593. Published 2021 Dec 20. doi:10.3390/cells10123593(IF:6.600)
[44] Jin T, Lin J, Gong Y, et al. iPLA2β Contributes to ER Stress-Induced Apoptosis during Myocardial Ischemia/Reperfusion Injury. Cells. 2021;10(6):1446. Published 2021 Jun 9. doi:10.3390/cells10061446(IF:6.600)
[45] Li H, Hou L, Zhang Y, et al. PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway. Cells. 2019;8(9):959. Published 2019 Aug 23. doi:10.3390/cells8090959(IF:6.600)
[46] Zhou X, Wang F, Wu H, et al. Thymoquinone Suppresses the Proliferation, Migration and Invasiveness through Regulating ROS, Autophagic Flux and miR-877-5p in Human Bladder Carcinoma Cells. Int J Biol Sci. 2021;17(13):3456-3475. Published 2021 Aug 12. doi:10.7150/ijbs.60401(IF:6.582)
[47] Wang B, Huang Y, Zhang Z, Xiao Y, Xie J. Ferulic Acid Treatment Maintains the Quality of Fresh-Cut Taro (Colocasia esculenta) During Cold Storage. Front Nutr. 2022;9:884844. Published 2022 May 24. doi:10.3389/fnut.2022.884844(IF:6.576)
[48] Deng Y, Li S, Chen Z, Wang W, Geng B, Cai J. Mdivi-1, a mitochondrial fission inhibitor, reduces angiotensin-II- induced hypertension by mediating VSMC phenotypic switch. Biomed Pharmacother. 2021;140:111689. doi:10.1016/j.biopha.2021.111689(IF:6.530)
[49] Li S, Hao M, Li B, et al. CACNA1H downregulation induces skeletal muscle atrophy involving endoplasmic reticulum stress activation and autophagy flux blockade. Cell Death Dis. 2020;11(4):279. Published 2020 Apr 24. doi:10.1038/s41419-020-2484-2(IF:6.304)
[50] Cai H, Li J, Zhang Y, et al. LDHA Promotes Oral Squamous Cell Carcinoma Progression Through Facilitating Glycolysis and Epithelial-Mesenchymal Transition. Front Oncol. 2019;9:1446. Published 2019 Dec 19. doi:10.3389/fonc.2019.01446(IF:6.244)
[51] Nan XW, Gong LH, Chen X, et al. Survivin Promotes Piperlongumine Resistance in Ovarian Cancer. Front Oncol. 2019;9:1345. Published 2019 Nov 29. doi:10.3389/fonc.2019.01345(IF:6.244)
[52] Zhou J, Wu L, Xu P, Li Y, Ji Z, Kang X. Filamin A Is a Potential Driver of Breast Cancer Metastasis via Regulation of MMP-1. Front Oncol. 2022;12:836126. Published 2022 Mar 11. doi:10.3389/fonc.2022.836126(IF:6.244)
[53] Wan Y, Hoyle RG, Xie N, et al. A Super-Enhancer Driven by FOSL1 Controls miR-21-5p Expression in Head and Neck Squamous Cell Carcinoma. Front Oncol. 2021;11:656628. Published 2021 Apr 16. doi:10.3389/fonc.2021.656628(IF:6.244)
[54] Wang F, Zhu Y, Cai H, et al. N6-Methyladenosine Methyltransferase METTL14-Mediated Autophagy in Malignant Development of Oral Squamous Cell Carcinoma. Front Oncol. 2021;11:738406. Published 2021 Nov 24. doi:10.3389/fonc.2021.738406(IF:6.244)
[55] Xu C, Zhang M, Bian L, Li Y, Yao Y, Li D. N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells. Oncogenesis. 2020;9(1):4. Published 2020 Jan 8. doi:10.1038/s41389-019-0188-1(IF:6.119)
[56] Zhu H, Zhang Y, Zhang C, Xie Z. RNA-Binding Profiles of CKAP4 as an RNA-Binding Protein in Myocardial Tissues. Front Cardiovasc Med. 2021;8:773573. Published 2021 Dec 23. doi:10.3389/fcvm.2021.773573(IF:6.050)
[57] Li M, Liu S, Tan L, et al. Fumonisin B1 induced intestinal epithelial barrier damage through endoplasmic reticulum stress triggered by the ceramide synthase 2 depletion. Food Chem Toxicol. 2022;166:113263. doi:10.1016/j.fct.2022.113263(IF:6.025)
[58] Zhai Y, Zheng X, Mao Y, et al. Recombinant Human Thymosin β4 (rhTβ4) Modulates the Anti-Inflammatory Responses to Alleviate Benzalkonium Chloride (BAC)-Induced Dry Eye Disease. Int J Mol Sci. 2022;23(10):5458. Published 2022 May 13. doi:10.3390/ijms23105458(IF:5.924)
[59] He G, Yang P, Cao Y, et al. Cytokinin Type-B Response Regulators Promote Bulbil Initiation in Lilium lancifolium. Int J Mol Sci. 2021;22(7):3320. Published 2021 Mar 24. doi:10.3390/ijms22073320(IF:5.924)
[60] Duan Z, Yan Q, Wu F, et al. Genome-Wide Analysis of the UDP-Glycosyltransferase Family Reveals Its Roles in Coumarin Biosynthesis and Abiotic Stress in Melilotus albus. Int J Mol Sci. 2021;22(19):10826. Published 2021 Oct 6. doi:10.3390/ijms221910826(IF:5.924)
[61] He G, Yang P, Cao Y, et al. Cytokinin Type-B Response Regulators Promote Bulbil Initiation in Lilium lancifolium. Int J Mol Sci. 2021;22(7):3320. Published 2021 Mar 24. doi:10.3390/ijms22073320(IF:5.924)
[62] Yuan L, Zhang L, Wei X, et al. Quantitative Trait Locus Mapping of Salt Tolerance in Wild Rice Oryza longistaminata. Int J Mol Sci. 2022;23(4):2379. Published 2022 Feb 21. doi:10.3390/ijms23042379(IF:5.924)
[63] Li P, Lan W, Li J, et al. Identification and Functional Evaluation of a Novel TBX4 Mutation Underlies Small Patella Syndrome. Int J Mol Sci. 2022;23(4):2075. Published 2022 Feb 14. doi:10.3390/ijms23042075(IF:5.924)
[64] Chen LJ, He JT, Pan M, et al. Antibiotics Attenuate Methamphetamine-Induced Hepatotoxicity by Regulating Oxidative Stress and TLR4/MyD88/Traf6 Axis. Front Pharmacol. 2021;12:716703. Published 2021 Jul 26. doi:10.3389/fphar.2021.716703(IF:5.811)
[65] Xing YJ, Liu BH, Wan SJ, et al. A SGLT2 Inhibitor Dapagliflozin Alleviates Diabetic Cardiomyopathy by Suppressing High Glucose-Induced Oxidative Stress in vivo and in vitro. Front Pharmacol. 2021;12:708177. Published 2021 Jul 12. doi:10.3389/fphar.2021.708177(IF:5.811)
[66] Yang Q, Nong X, Xu J, et al. Unraveling the Genetic Basis of Fertility Restoration for Cytoplasmic Male Sterile Line WNJ01A Originated From Brassica juncea in Brassica napus. Front Plant Sci. 2021;12:721980. Published 2021 Aug 31. doi:10.3389/fpls.2021.721980(IF:5.754)
[67] Liao Y, Wang F, Zhang Y, Cai H, Song F, Hou J. Silencing SHMT2 inhibits the progression of tongue squamous cell carcinoma through cell cycle regulation. Cancer Cell Int. 2021;21(1):220. Published 2021 Apr 16. doi:10.1186/s12935-021-01880-5(IF:5.722)
[68] Ding FP, Tian JY, Wu J, Han DF, Zhao D. Identification of key genes as predictive biomarkers for osteosarcoma metastasis using translational bioinformatics. Cancer Cell Int. 2021;21(1):640. Published 2021 Dec 2. doi:10.1186/s12935-021-02308-w(IF:5.722)
[69] Miao D, Shi J, Xiong Z, et al. As a prognostic biomarker of clear cell renal cell carcinoma RUFY4 predicts immunotherapy responsiveness in a PDL1-related manner. Cancer Cell Int. 2022;22(1):66. Published 2022 Feb 8. doi:10.1186/s12935-022-02480-7(IF:5.722)
[70] Fan X, Li Y, Yi X, et al. Epigenome-wide DNA methylation profiling of portal vein tumor thrombosis (PVTT) tissues in hepatocellular carcinoma patients. Neoplasia. 2020;22(11):630-643. doi:10.1016/j.neo.2020.09.007(IF:5.696)
[71] Zhu Z, Zhang Y, Wang X, Wang X, Ye SD. Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells. Development. 2020;147(16):dev185264. Published 2020 Aug 24. doi:10.1242/dev.185264(IF:5.611)
[72] Zhang C, Shen Y, Tang D, et al. The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice. PLoS Genet. 2018;14(11):e1007769. Published 2018 Nov 12. doi:10.1371/journal.pgen.1007769(IF:5.540)
[73] Sui Q, Chen Z, Hu Z, et al. Cisplatin resistance-related multi-omics differences and the establishment of machine learning models. J Transl Med. 2022;20(1):171. Published 2022 Apr 11. doi:10.1186/s12967-022-03372-0(IF:5.531)
[74] Qiu X, Wang W, Zhang L, Guo L, Xu P, Tang H. A thermophile Hydrogenibacillus sp. strain efficiently degrades environmental pollutants polycyclic aromatic hydrocarbons. Environ Microbiol. 2022;24(1):436-450. doi:10.1111/1462-2920.15869(IF:5.491)
[75] Li J, Yang YL, Li LZ, et al. Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1. Biochim Biophys Acta Mol Basis Dis. 2017;1863(11):2835-2847. doi:10.1016/j.bbadis.2017.07.017(IF:5.476)
[76] Zhao Y, Sun J, Li Y, et al. The strand-biased transcription of SARS-CoV-2 and unbalanced inhibition by remdesivir. iScience. 2021;24(8):102857. doi:10.1016/j.isci.2021.102857(IF:5.458)
[77] Chen L, Ma Q, Zhang G, et al. Protective effect and mechanism of loganin and morroniside on acute lung injury and pulmonary fibrosis [published online ahead of print, 2022 Mar 5]. Phytomedicine. 2022;99:154030. doi:10.1016/j.phymed.2022.154030(IF:5.340)
[78] Chen L, Kan J, Zheng N, et al. A botanical dietary supplement from white peony and licorice attenuates nonalcoholic fatty liver disease by modulating gut microbiota and reducing inflammation. Phytomedicine. 2021;91:153693. doi:10.1016/j.phymed.2021.153693(IF:5.340)
[79] Wang Z, Nie K, Su H, et al. Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome. Phytomedicine. 2021;91:153654. doi:10.1016/j.phymed.2021.153654(IF:5.340)
[80] Wang M, Guo W, Li J, et al. The miR528-AO Module Confers Enhanced Salt Tolerance in Rice by Modulating the Ascorbic Acid and Abscisic Acid Metabolism and ROS Scavenging. J Agric Food Chem. 2021;69(31):8634-8648. doi:10.1021/acs.jafc.1c01096(IF:5.279)
[81] Li N, Chen J, Geng C, et al. Myoglobin promotes macrophage polarization to M1 type and pyroptosis via the RIG-I/Caspase1/GSDMD signaling pathway in CS-AKI. Cell Death Discov. 2022;8(1):90. Published 2022 Feb 28. doi:10.1038/s41420-022-00894-w(IF:5.241)
[82] Zhang X, Li Y, Ji J, et al. Gadd45g initiates embryonic stem cell differentiation and inhibits breast cell carcinogenesis. Cell Death Discov. 2021;7(1):271. Published 2021 Oct 2. doi:10.1038/s41420-021-00667-x(IF:5.241)
[83] Kang H, Guo Q, Dong Y, et al. Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss. FASEB J. 2022;36(2):e22167. doi:10.1096/fj.202101205RR(IF:5.192)
[84] Shen Y, Xu LR, Yan D, et al. BMAL1 modulates smooth muscle cells phenotypic switch towards fibroblast-like cells and stabilizes atherosclerotic plaques by upregulating YAP1. Biochim Biophys Acta Mol Basis Dis. 2022;1868(9):166450. doi:10.1016/j.bbadis.2022.166450(IF:5.187)
[85] Zhang M, Ji J, Wang X, et al. The transcription factor Tfcp2l1 promotes primordial germ cell-like cell specification of pluripotent stem cells. J Biol Chem. 2021;297(4):101217. doi:10.1016/j.jbc.2021.101217(IF:5.157)
[86] He L, Gao K, Liu H, Wang J, Li X, He C. Smooth muscle cell-specific knockout of interferon gamma (IFN-γ) receptor attenuates intimal hyperplasia via STAT1-KLF4 activation [published online ahead of print, 2021 May 25]. Life Sci. 2021;119651. doi:10.1016/j.lfs.2021.119651(IF:5.037)
[87] Yang L, Wang X, Jiao X, et al. Suppressor of Ty 16 promotes lung cancer malignancy and is negatively regulated by miR-1227-5p. Cancer Sci. 2020;111(11):4075-4087. doi:10.1111/cas.14627(IF:4.966)
[88] Hu R, Zhu X, Chen C, Xu R, Li Y, Xu W. RNA-binding protein PUM2 suppresses osteosarcoma progression via partly and competitively binding to STARD13 3'UTR with miRNAs. Cell Prolif. 2018;51(6):e12508. doi:10.1111/cpr.12508(IF:4.936)
[89] Li Z, Wang Y, Hu R, Xu R, Xu W. LncRNA B4GALT1-AS1 recruits HuR to promote osteosarcoma cells stemness and migration via enhancing YAP transcriptional activity. Cell Prolif. 2018;51(6):e12504. doi:10.1111/cpr.12504(IF:4.936)
[90] Zhou M, Li B, Liu C, et al. M2 Macrophage-derived exosomal miR-501 contributes to pubococcygeal muscle regeneration. Int Immunopharmacol. 2021;101(Pt B):108223. doi:10.1016/j.intimp.2021.108223(IF:4.932)
[91] Li S, Wang D, Wei P, et al. Elevated Natural Killer Cell-Mediated Cytotoxicity Is Associated with Cavity Formation in Pulmonary Tuberculosis Patients. J Immunol Res. 2021;2021:7925903. Published 2021 Oct 4. doi:10.1155/2021/7925903(IF:4.818)
[92] Zhou X , Weng W , Chen B , et al. Mesoporous silica nanoparticles/gelatin porous composite scaffolds with localized and sustained release of vancomycin for treatment of infected bone defects. J Mater Chem B. 2018;6(5):740-752. doi:10.1039/c7tb01246b(IF:4.776)
[93] Yang H, Mei W, Wan H, Xu R, Cheng Y. Comprehensive analysis of KCS gene family in Citrinae reveals the involvement of CsKCS2 and CsKCS11 in fruit cuticular wax synthesis at ripening. Plant Sci. 2021;310:110972. doi:10.1016/j.plantsci.2021.110972(IF:4.729)
[94] He B, Kang S, Chen Z, et al. Hypercholesterolemia risk associated Abca6 does not regulate lipoprotein metabolism in mice or hamster. Biochim Biophys Acta Mol Cell Biol Lipids. 2021;1866(11):159006. doi:10.1016/j.bbalip.2021.159006(IF:4.698)
[95] Yang H, Zhu Z, Zhang M, et al. CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit. Plant Cell Rep. 2022;41(4):905-920. doi:10.1007/s00299-021-02826-x(IF:4.570)
[96] Sun J, Ha N, Liu Z, Bian Q, Wang X. A Neural Crest-specific Overexpression Mouse Model Reveals the Transcriptional Regulatory Effects of Dlx2 During Maxillary Process Development. Front Physiol. 2022;13:855959. Published 2022 Apr 21. doi:10.3389/fphys.2022.855959(IF:4.566)
[97] Zhang L, Dong Y, Xue Y, et al. Multifunctional Triple-Layered Composite Scaffolds Combining Platelet-Rich Fibrin Promote Bone Regeneration. ACS Biomater Sci Eng. 2019;5(12):6691-6702. doi:10.1021/acsbiomaterials.9b01022(IF:4.511)
[98] Wang T, Cheng C, Peng L, et al. Combination of arsenic trioxide and Dasatinib: a new strategy to treat Philadelphia chromosome-positive acute lymphoblastic leukaemia. J Cell Mol Med. 2018;22(3):1614-1626. doi:10.1111/jcmm.13436(IF:4.499)
[99] Song D, Gui J, Liu C, Sun J, Li L. Suppression of PtrDUF579-3 Expression Causes Structural Changes of the Glucuronoxylan in Populus. Front Plant Sci. 2016;7:493. Published 2016 Apr 11. doi:10.3389/fpls.2016.00493(IF:4.495)
[100] Li J, Chen YH, Li LZ, et al. Omics and Transgenic Analyses Reveal that Salvianolic Acid B Exhibits its Anti-Inflammatory Effects through Inhibiting the Mincle-Syk-Related Pathway in Macrophages. J Proteome Res. 2021;20(7):3734-3748. doi:10.1021/acs.jproteome.1c00325(IF:4.466)
[101] Xu Y, Cai Z, Ba L, et al. Maintenance of Postharvest Quality and Reactive Oxygen Species Homeostasis of Pitaya Fruit by Essential Oil p-Anisaldehyde Treatment. Foods. 2021;10(10):2434. Published 2021 Oct 13. doi:10.3390/foods10102434(IF:4.350)
[102] Peng O, Wei X, Ashraf U, et al. Genome-wide transcriptome analysis of porcine epidemic diarrhea virus virulent or avirulent strain-infected porcine small intestinal epithelial cells. Virol Sin. 2022;37(1):70-81. doi:10.1016/j.virs.2022.01.011(IF:4.327)
[103] Li H, Zhao Z, Ling J, et al. USP14 promotes K63-linked RIG-I deubiquitination and suppresses antiviral immune responses. Eur J Immunol. 2019;49(1):42-53. doi:10.1002/eji.201847603(IF:4.248)
[104] Zhang P, Li H, Zhou C, et al. Single-Cell RNA Transcriptomics Reveals the State of Hepatic Lymphatic Endothelial Cells in Hepatitis B Virus-Related Acute-on-Chronic Liver Failure. J Clin Med. 2022;11(10):2910. Published 2022 May 20. doi:10.3390/jcm11102910(IF:4.242)
[105] Ge S, Zhang Q, Chen Y, et al. Ribavirin inhibits colorectal cancer growth by downregulating PRMT5 expression and H3R8me2s and H4R3me2s accumulation. Toxicol Appl Pharmacol. 2021;415:115450. doi:10.1016/j.taap.2021.115450(IF:4.219)
[106] Bai RB, Zhang YJ, Fan JM, et al. Immune-enhancement effects of oligosaccharides from Codonopsis pilosula on cyclophosphamide induced immunosuppression in mice. Food Funct. 2020;11(4):3306-3315. doi:10.1039/c9fo02969a(IF:4.171)
[107] Zhou Y, Zhu Y, Dong X, et al. Exosomes Derived from Pancreatic Cancer Cells Induce Osteoclast Differentiation Through the miR125a-5p/TNFRSF1B Pathway. Onco Targets Ther. 2021;14:2727-2739. Published 2021 Apr 19. doi:10.2147/OTT.S282319(IF:4.147)
[108] Yan H, Li Q, Li M, et al. Ajuba functions as a co-activator of C/EBPβ to induce expression of PPARγ and C/EBPα during adipogenesis. Mol Cell Endocrinol. 2022;539:111485. doi:10.1016/j.mce.2021.111485(IF:4.102)
[109] Liu F, Xia Z, Zhang M, et al. SMARCAD1 Promotes Pancreatic Cancer Cell Growth and Metastasis through Wnt/β-catenin-Mediated EMT. Int J Biol Sci. 2019;15(3):636-646. Published 2019 Jan 1. doi:10.7150/ijbs.29562(IF:4.067)
[110] Ma X, Gao Y, Liu J, et al. Low expression of PCK2 in breast tumors contributes to better prognosis by inducing senescence of cancer cells [published online ahead of print, 2022 May 17]. IUBMB Life. 2022;10.1002/iub.2651. doi:10.1002/iub.2651(IF:3.885)
[111] Li H, Chen W, Chen Y, et al. Neferine Attenuates Acute Kidney Injury by Inhibiting NF-κB Signaling and Upregulating Klotho Expression. Front Pharmacol. 2019;10:1197. Published 2019 Oct 15. doi:10.3389/fphar.2019.01197(IF:3.845)
[112] He G, Yang P, Tang Y, et al. Mechanism of exogenous cytokinins inducing bulbil formation in Lilium lancifolium in vitro. Plant Cell Rep. 2020;39(7):861-872. doi:10.1007/s00299-020-02535-x(IF:3.825)
[113] Zhang J, Wang W, Zhu S, Chen Y. Increased SERPINA3 Level Is Associated with Ulcerative Colitis. Diagnostics (Basel). 2021;11(12):2371. Published 2021 Dec 16. doi:10.3390/diagnostics11122371(IF:3.706)
[114] Li H, Quan J, Zhao X, Ling J, Chen W. USP14 negatively regulates RIG-I-mediated IL-6 and TNF-α production by inhibiting NF-κB activation. Mol Immunol. 2021;130:69-76. doi:10.1016/j.molimm.2020.12.022(IF:3.641)
[115] Hao Y, Huang J, Ran Y, et al. Ethylmalonic encephalopathy 1 initiates overactive autophagy in depleted uranium-induced cytotoxicity in the human embryonic kidney 293 cells. J Biochem Mol Toxicol. 2021;35(3):e22669. doi:10.1002/jbt.22669(IF:3.606)
[116] Huang L, He S, Cai Q, et al. Polydatin alleviates traumatic brain injury: Role of inhibiting ferroptosis. Biochem Biophys Res Commun. 2021;556:149-155. doi:10.1016/j.bbrc.2021.03.108(IF:3.575)
[117] You Y, Cui Y, Li Y, et al. Inhibition of MTA2 and MTA3 induces mesendoderm specification of human embryonic stem cells. Biochem Biophys Res Commun. 2021;552:142-149. doi:10.1016/j.bbrc.2021.03.030(IF:3.575)
[118] Li M, Ning N, Liu Y, et al. The potential of Zishen Yutai pills to facilitate endometrial recovery and restore fertility after induced abortion in rats. Pharm Biol. 2021;59(1):1505-1516. doi:10.1080/13880209.2021.1993272(IF:3.503)
[119] Zhang H, Chen M, Wen H, et al. Transcriptomic and metabolomic analyses provide insight into the volatile compounds of citrus leaves and flowers. BMC Plant Biol. 2020;20(1):7. Published 2020 Jan 6. doi:10.1186/s12870-019-2222-z(IF:3.497)
[120] Du Q, Zhang S, Li A, Mohammad IS, Liu B, Li Y. Astragaloside IV Inhibits Adipose Lipolysis and Reduces Hepatic Glucose Production via Akt Dependent PDE3B Expression in HFD-Fed Mice. Front Physiol. 2018;9:15. Published 2018 Jan 23. doi:10.3389/fphys.2018.00015(IF:3.394)
[121] Yang Y, Huang C, Lin X, et al. 0.005% Preservative-Free Latanoprost Induces Dry Eye-Like Ocular Surface Damage via Promotion of Inflammation in Mice. Invest Ophthalmol Vis Sci. 2018;59(8):3375-3384. doi:10.1167/iovs.18-24013(IF:3.388)
[122] Xiang Y, Yu Y, Li Q, Chen J, Li Y, Cao W. Chicken telomerase reverse transcriptase mediates LMH cell pyroptosis by regulating the nuclear factor-kappa B signaling pathway. Poult Sci. 2022;101(6):101826. doi:10.1016/j.psj.2022.101826(IF:3.352)
[123] Wang PT, Li N, Wang XY, et al. RIG-I, a novel DAMPs sensor for myoglobin activates NF-κB/caspase-3 signaling in CS-AKI model. Mil Med Res. 2021;8(1):37. Published 2021 Jun 21. doi:10.1186/s40779-021-00333-4(IF:3.329)
[124] Chen S, Li M, Jiang W, Zheng H, Qi LW, Jiang S. The role of Neu1 in the protective effect of dipsacoside B on acetaminophen-induced liver injury. Ann Transl Med. 2020;8(13):823. doi:10.21037/atm-19-3850(IF:3.297)
[125] Xue X, Chen Y. Circular RNA (circ)_0129047 upregulates bone morphogenetic protein receptor type 2 expression to inhibit lung adenocarcinoma progression by sponging microRNA (miR)-1206. Bioengineered. 2022;13(5):12067-12087. doi:10.1080/21655979.2022.2070580(IF:3.269)
[126] Zhuo Y, Guo Z, Ba T, et al. African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. Virol Sin. 2021;36(2):176-186. doi:10.1007/s12250-020-00304-4(IF:3.242)
[127] Rothenberg DO, Yang H, Chen M, Zhang W, Zhang L. Metabolome and Transcriptome Sequencing Analysis Reveals Anthocyanin Metabolism in Pink Flowers of Anthocyanin-Rich Tea (Camellia sinensis). Molecules. 2019;24(6):1064. Published 2019 Mar 18. doi:10.3390/molecules24061064(IF:3.060)
[128] Zhang Y, Zhu Z, Ding H, et al. β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells. Biochem Biophys Res Commun. 2020;524(2):280-287. doi:10.1016/j.bbrc.2020.01.074(IF:2.985)
[129] Liu X, Yang X, Zhang B. Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress. PeerJ. 2021;9:e12492. Published 2021 Nov 11. doi:10.7717/peerj.12492(IF:2.984)
[130] Liu Z, Wang Y, Qin W, et al. Raloxifene alleviates amyloid-β-induced cytotoxicity in HT22 neuronal cells via inhibiting oligomeric and fibrillar species formation. J Biochem Mol Toxicol. 2019;33(11):e22395. doi:10.1002/jbt.22395(IF:2.965)
[131] Zheng C, Ouyang YC, Jiang B, et al. Non-canonical RNA polyadenylation polymerase FAM46C is essential for fastening sperm head and flagellum in mice†. Biol Reprod. 2019;100(6):1673-1685. doi:10.1093/biolre/ioz083(IF:2.960)
[132] Wang Z, Xu J, Liu Y, et al. Selection and validation of appropriate reference genes for real-time quantitative PCR analysis in Momordica charantia. Phytochemistry. 2019;164:1-11. doi:10.1016/j.phytochem.2019.04.010(IF:2.905)
[133] Liu L, Yang Y, Li W, Li Y, Jiang X, Wang L. Tanshinone IIA alleviate rifampicin-induced cholestasis by regulating the expression and function of bile salt export pump. Hum Exp Toxicol. 2022;41:9603271221097365. doi:10.1177/09603271221097365(IF:2.903)
[134] Xu WH, Liang DY, Wang Q, Shen J, Liu QH, Peng YB. Knockdown of KDM2A inhibits proliferation associated with TGF-β expression in HEK293T cell. Mol Cell Biochem. 2019;456(1-2):95-104. doi:10.1007/s11010-018-03493-5(IF:2.884)
[135] Xiao Q, Dong ZQ, Zhu Y, et al. Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1. Insects. 2021;12(12):1098. Published 2021 Dec 8. doi:10.3390/insects12121098(IF:2.769)
[136] Wang Z, Dong H, Yang L, Yi P, Wang Q, Huang D. The role of FDX1 in granulosa cell of Polycystic ovary syndrome (PCOS). BMC Endocr Disord. 2021;21(1):119. Published 2021 Jun 15. doi:10.1186/s12902-021-00775-w(IF:2.763)
[137] Liu J, Guo M, Fan X. Ethanol induces necroptosis in gastric epithelial cells in vitro. J Food Biochem. 2021;45(4):e13692. doi:10.1111/jfbc.13692(IF:2.720)
[138] Tan D, Hu H, Tong X, et al. Genome-wide identification and characterization of myosin genes in the silkworm, Bombyx mori. Gene. 2019;691:45-55. doi:10.1016/j.gene.2018.12.011(IF:2.638)
[139] Chen X, Liu Y, Meng B, Wu D, Wu Y, Cao Y. Interleukin-20 inhibits the osteogenic differentiation of MC3T3-E1 cells via the GSK3β/β-catenin signalling pathway. Arch Oral Biol. 2021;125:105111. doi:10.1016/j.archoralbio.2021.105111(IF:2.635)
[140] Huang R, Dong R, Wang N, Lan B, Zhao H, Gao Y. Exploring the Antiglioma Mechanisms of Luteolin Based on Network Pharmacology and Experimental Verification. Evid Based Complement Alternat Med. 2021;2021:7765658. Published 2021 Nov 27. doi:10.1155/2021/7765658(IF:2.630)
[141] Huang J, Guo M, Jin S, et al. Antibacterial photodynamic therapy mediated by 5-aminolevulinic acid on methicillin-resistant Staphylococcus aureus. Photodiagnosis Photodyn Ther. 2019;28:330-337. doi:10.1016/j.pdpdt.2019.09.008(IF:2.589)
[142] Duan RS, Liu PP, Xi F, et al. Wnt3 and Gata4 regulate axon regeneration in adult mouse DRG neurons. Biochem Biophys Res Commun. 2018;499(2):246-252. doi:10.1016/j.bbrc.2018.03.138(IF:2.559)
[143] Guo Z, Qiu C, Mecca C, et al. Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration. BMC Musculoskelet Disord. 2021;22(1):77. Published 2021 Jan 13. doi:10.1186/s12891-020-03934-7(IF:2.355)
[144] You L, Chen H, Xu L, Li X. Overexpression of miR-29a-3p Suppresses Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells in Atherosclerosis via Targeting TNFRSF1A. Biomed Res Int. 2020;2020:9627974. Published 2020 Sep 4. doi:10.1155/2020/9627974(IF:2.276)
[145] Chen G, Yang Z, Wen D, et al. Polydatin has anti-inflammatory and antioxidant effects in LPS-induced macrophages and improves DSS-induced mice colitis. Immun Inflamm Dis. 2021;9(3):959-970. doi:10.1002/iid3.455(IF:2.239)
[146] Chen M, Li S, Hao M, et al. T-type calcium channel blockade induces apoptosis in C2C12 myotubes and skeletal muscle via endoplasmic reticulum stress activation. FEBS Open Bio. 2020;10(10):2122-2136. doi:10.1002/2211-5463.12965(IF:2.231)
[147] Zhang Y, Zhou S, Cai W, et al. Hypoxia/reoxygenation activates the JNK pathway and accelerates synovial senescence. Mol Med Rep. 2020;22(1):265-276. doi:10.3892/mmr.2020.11102(IF:2.100)
[148] Zhou F, Liu P, Lv H, Gao Z, Chang W, Xu Y. miR-31 attenuates murine allergic rhinitis by suppressing interleukin-13-induced nasal epithelial inflammatory responses. Mol Med Rep. 2021;23(1):42. doi:10.3892/mmr.2020.11680(IF:2.100)
[149] Wu H, He Y, Chen H, et al. LncRNA THOR increases osteosarcoma cell stemness and migration by enhancing SOX9 mRNA stability. FEBS Open Bio. 2019;9(4):781-790. Published 2019 Mar 20. doi:10.1002/2211-5463.12620(IF:1.959)
[150] Liu X, Xie F, Lai G, Wang J. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Arch Oral Biol. 2020;120:104933. doi:10.1016/j.archoralbio.2020.104933(IF:1.931)
[151] Yi S, Song X, Yu W, et al. De novo assembly and Transcriptome Analysis of the Momordica charantia Seedlings Responding to methyl jasmonate using 454 pyrosequencing. Gene Expr Patterns. 2021;40:119160. doi:10.1016/j.gep.2020.119160(IF:0.897)
