Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody?dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells and associates with Fc R gamma to deliver an activating signal upon ligand binding.

 

產(chǎn)品性質(zhì)

別名	IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20
Uniprot No.	Q5D5I8
表達(dá)區(qū)間及表達(dá)系統(tǒng)	Recombinant Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Leu32-Thr215.
分子量	Approximately 22.2 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	SPR Data：OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIII, His Tag with an affinity constant of 0.194 μM as determined in SPR assay (Biacore T200).
內(nèi)毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
復(fù)溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

冰袋運輸。-20℃至-80℃保存，一年有效期。

復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復(fù)凍融。

 

注意事項

1. 避免反復(fù)凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產(chǎn)品僅作科研用途！

 

產(chǎn)品數(shù)據(jù)

HB220811