4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine?rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. 4?1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity. It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome. Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively.  

 

產(chǎn)品性質(zhì)

別名	CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Uniprot No.	Q07011
表達區(qū)間及表達系統(tǒng)	Recombinant Biotinylated Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Leu24-Gln186.
分子量	Approximately 20.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data: Immobilized Human 4-1BB Ligand Trimer, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human 4-1BB, His Tag with the EC50 of 0.58μg/ml determined by ELISA.
內(nèi)毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
復(fù)溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

冰袋運輸。-20℃至-80℃保存，一年有效期。

復(fù)溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復(fù)溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復(fù)凍融。

 

注意事項

 

1. 避免反復(fù)凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產(chǎn)品僅作科研用途！

 

產(chǎn)品數(shù)據(jù)
 

HB220805