4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts. It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells. This interaction co?stimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell. It can also enhance the activation-induced cell death of repetitively stimulated T cells. 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation.

 

產品性質

別名	CD137; TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CDw137; FLJ43501; ILA; T cell antigen ILA
Uniprot No.	Q07011
表達區(qū)間及表達系統(tǒng)	Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from HEK293 Cells with hFc tag and Avi tag at the C-terminal. It contains Leu24-Gln186.
分子量	Approximately 45.7 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
純度	> 95% as determined by SDS-PAGE and HPLC.
活性	ELISA Data: Immobilized Human 4-1BB Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
內毒素	< 1.0 EU per 1μg of the protein by the LAL method.
制劑	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
復溶	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

運輸與保存方法

冰袋運輸。-20℃至-80℃保存，一年有效期。

復溶后，-20 至 -80°C，在未開封狀態(tài)下保存 3-6 個月。復溶后，2-8°C 保存 2-7 天。

建議第一次使用時分裝凍存，避免反復凍融。

注意事項

1. 避免反復凍融。

2. 為了您的安全和健康，請穿實驗服并戴一次性手套操作。

3. 本產品僅作科研用途！

 

產品數(shù)據(jù)
 

HB220803